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| Product Name | Ready-to-Use ssDNA Quantitation Kit (1x premix, 0-200 ng) |
| Catalog No. | REKI-0098 |
| Description | A premixed single-stranded DNA quantitation assay delivered at working strength: no preparation steps stand between sample and readout on a fluorometer or plate reader. The assay is linear across 0 to 200 ng with particularly good behaviour below 10 ng, and detection reaches 50 pg/uL. Stability data show no meaningful performance change after nine months at 4 or 25 °C, and the chemistry tolerates proteins, salts, free nucleotides, solvents and detergents, covering ssDNA libraries, circularisation products, oligonucleotides and denatured DNA. |
| Intended Use | Quantification of ssDNA libraries, circularised products, oligonucleotides, primers and denatured DNA in sequencing workflows. |
| Principle / Technology | A ssDNA-responsive fluorescent dye premixed at working strength reports sample concentration directly. |
| Sample Type | Single-stranded DNA, oligonucleotides, primers, denatured DNA, single-stranded circular DNA |
| Performance Range / Specifications | Linear 0 to 200 ng with strong low-end behaviour; detection limit 50 pg/uL; nine-month stability at 4 and 25 °C. |
| Specificity | Reported for ssDNA targets; broad contaminant tolerance. |
| Reaction Conditions / Protocol | Mix the ready premix with standards and samples and read fluorescence directly. |
| Components / Formulation | 1x working-strength fluorescence reagent with ssDNA standards. |
| Storage Conditions | 2 to 8 °C protected from light |
| Shelf Life | 12 months |
| Package Specifications | 100 T, 500 T |
| Product Form | Ready-to-use premix |
| Key Features | Zero-prep workflow; strong low-concentration linearity; long ambient stability. |
| Shipping Conditions | Cold pack shipping; avoid repeated freeze-thaw |
| Compatibility | Fluorometers and fluorescence plate readers |
| Readouts / Downstream Assays | ssDNA concentration for library workflows |
For research use only, not for clinical use.
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