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| Product Name | Rapid One-Tube End Repair and A-Tailing Module (cfDNA and FFPE compatible) |
| Catalog No. | REKI-0086 |
| Description | A fast end repair and A-tailing module built for sequencing library construction on short-read platforms. In a single tube, fragmented input from 1 ng to 1 ug is end polished, 5-prime phosphorylated and extended with a 3-prime dA overhang, producing ligation-ready molecules without intermediate cleanup. The streamlined, automation-friendly reaction suits demanding inputs such as circulating cell-free DNA and archival FFPE material. |
| Intended Use | End repair and A-tailing of cfDNA, FFPE DNA and mechanically or enzymatically fragmented DNA before adapter ligation. |
| Principle / Technology | Combined polymerase and phosphatase activities polish ends, add 5-prime phosphates and generate 3-prime A overhangs in one reaction. |
| Sample Type | cfDNA, FFPE DNA, fragmented DNA |
| Performance Range / Specifications | Input 1 ng to 1 ug; single-tube workflow; automation compatible. |
| Specificity | Tuned for rapid processing of low-input and degraded templates. |
| Reaction Conditions / Protocol | Mix fragmented DNA with the module, incubate, then carry the product straight to adapter ligation. |
| Components / Formulation | End repair and A-tailing enzyme mix with reaction buffer. |
| Storage Conditions | -25 to -15 °C |
| Shelf Life | 1 year |
| Package Specifications | 24 T, 96 T |
| Product Form | Enzyme and buffer premix |
| Key Features | One-tube speed; cfDNA and FFPE focus; ligation-ready output without cleanup. |
| Compatibility | Short-read sequencing library workflows; automation platforms |
| Starting Material / Tissue Site | 1 ng to 1 ug fragmented DNA |
| Readouts / Downstream Assays | Ligation-ready library DNA |
For research use only, not for clinical use.
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