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| Product Name | Rapid DpnI Restriction Endonuclease |
| Catalog No. | IEC-HMM-0087 |
| Description | A recombinant rapid-digestion restriction endonuclease formulated for efficient cleavage of genomic DNA, plasmid DNA and PCR products in a universal buffer system. |
| Intended Use | Restriction digestion of genomic DNA, plasmid DNA and PCR products. |
| Principle / Technology | The recombinant restriction endonuclease recognizes its specific DNA target and cleaves the phosphodiester backbone in a rapid universal-buffer system. |
| Sample Type | Genomic DNA; plasmid DNA; PCR products. |
| Performance Range / Specifications | Rapid digestion in as little as 15 minutes; full activity in the universal rapid digestion buffer. |
| Specificity | Selectively recognizes methylated or hemimethylated GATC sites and cleaves DNA containing N6-methyladenine. |
| Reaction Conditions / Protocol | Incubate the DNA substrate with the enzyme in 1× universal rapid digestion buffer for approximately 15 minutes. |
| Components / Formulation | Recombinant DpnI Restriction Endonuclease preparation. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 50 reactions |
| Product Form | Rapid restriction endonuclease solution. |
| Quality Control | Activity is verified in the universal rapid digestion buffer. |
| Key Features | Fast digestion; universal buffer compatibility; suitable for genomic DNA, plasmids and PCR products; optional colored loading buffer enables direct gel loading. |
| Shipping Conditions | Ship at or below 0°C. |
| Compatibility | Compatible with the universal rapid digestion buffer and colored universal rapid digestion buffer. |
| Recommended Buffer System | 10× universal rapid digestion buffer or 10× colored universal rapid digestion buffer. |
| Application Notes / Precautions | When the colored buffer is used, the reaction can be loaded directly onto an agarose gel. The red tracking dye migrates near a 2,500 bp double-stranded DNA fragment in 1% agarose, while the yellow dye migrates near a 10 bp fragment. |
| Recognition Sequence / Cleavage Site | 5′-Gm6ATC-3′; cleaves methylated or hemimethylated GATC sites. |
For research use only, not for clinical use.
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