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| Product Name | Quick Exosome Isolation Kit Plus (for Serum/Plasma) |
| Catalog No. | RESK-0611 |
| Description | Exosomes are small vesicles approximately 30-150 nm in diameter secreted by living cells, with a typical lipid bilayer structure. They are present in cell culture supernatant, serum, plasma, saliva, urine, amniotic fluid, and other biological fluids. Exosomes carry important cargo such as proteins, lipids, DNA, and RNA; they not only play important roles in intercellular material and information transfer, but are also promising early diagnostic biomarkers for a variety of diseases. This kit uses a unique isolation technology to rapidly obtain large amounts of intact exosome particles from serum/plasma. The isolated exosomes can be used for NTA particle size analysis, Western blot, nucleic acid extraction and downstream analysis (qPCR, sequencing, etc.); they are not recommended for exosome labeling or protein mass spectrometry analysis. |
| Intended Use | Rapid isolation and purification of intact exosomes from serum or plasma for downstream research applications. For research use only. |
| Principle / Technology | Co-precipitation-based exosome enrichment combined with spin column purification: the serum/plasma sample is mixed with the sample solution and the exosome extraction reagent, incubated at 4 °C to precipitate exosomes, collected by centrifugation, resuspended, and purified on a filter spin column (a 220 nm filter column that removes large-particle impurities). |
| Detection Method | Isolated exosomes can be analyzed by NTA particle size analysis, Western blot, and nucleic acid extraction followed by qPCR or sequencing. |
| Sample Type | Serum or plasma (fresh or frozen). |
| Performance Range / Specifications | Sample input per reaction: 250 uL of pretreated serum or plasma (minimum 100 uL), mixed with an equal volume of sample solution and 125 uL of exosome extraction reagent. Isolated exosomes can be stored at 2-8 °C for one week or at -80 °C for approximately three months. |
| Specificity | Designed specifically for exosome isolation from serum/plasma samples. |
| Reaction Conditions / Protocol | 1. Sample pretreatment 1) For frozen samples, thaw at room temperature or in a 25 °C water bath and place the completely melted sample on ice. 2) For fresh samples, keep on ice after collection, centrifuge at 3,000 x g for 15 min at 4 °C to remove cells or cell debris, and transfer the supernatant to a new tube. 2. Exosome precipitation 1) Pipette 250 uL (at least 100 uL) of pretreated serum or plasma into a 1.5 mL centrifuge tube (not provided), add an equal volume (250 uL) of sample solution and mix, then add 125 uL of exosome extraction reagent, mix thoroughly by inversion, and let stand at 4 °C for at least 30 min. 2) Centrifuge at 10,000 x g for 30 min at 4 °C; a white pellet is visible at the bottom of the tube. Aspirate the supernatant, then centrifuge at 10,000 x g for 5 min at 4 °C to completely remove residual liquid, taking care not to disturb the exosome pellet. 3. Exosome resuspension Add 100 uL of sample solution, or the buffer required for the downstream application, and pipette repeatedly or vortex to completely dissolve the pellet; this resuspension contains the extracted intact exosomes. 4. Exosome purification Transfer the resuspended exosomes to a purification spin column placed in a 1.5 mL collection tube (provided) and centrifuge at 2,000 x g for 5 min at 4 °C; discard the spin column. The collection tube contains the extracted exosomes, which can be used directly in downstream experiments (e.g., particle size analysis, nucleic acid extraction) or stored at 2-8 °C for one week or at -80 °C for approximately three months. |
| Components / Formulation | Exosome Extraction Reagent: 15 mL (store at 2-8 °C) Sample Solution: 30 mL (store at 2-8 °C) Purification spin columns (filter spin columns) and 1.5 mL collection tubes: 30 tubes (store at room temperature) |
| Storage Conditions | Store at room temperature or 2-8 °C according to the individual components; mix thoroughly before use. |
| Shelf Life | 12 months when stored as recommended. |
| Package Specifications | 30 T 2 T |
| Product Form | Liquid reagents with single-use spin columns and collection tubes. |
| Shipping Conditions | Shipped at room temperature. |
| Expiration Date / Stability | Stable for 12 months when stored at room temperature or 2-8 °C as recommended for each component. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Isolated exosomes are suitable for NTA particle size analysis, Western blot, nucleic acid extraction and downstream analysis (qPCR, sequencing). Not recommended for exosome labeling or protein mass spectrometry analysis. |
| Recommended Buffer System | Resuspend the exosome pellet in the provided sample solution or in the buffer required by the downstream application. |
| Application Notes / Precautions | This kit uses a co-precipitation method. For proteomics applications, a kit version with an additional contaminating-protein removal step is recommended, as it delivers higher exosome purity and less interference with downstream experiments (the protein concentration obtained from the same sample may be lower, but the purity is higher). The purification column is a 220 nm filter column that removes large-particle impurities. Mix the reagents thoroughly before use. |
For research use only, not for clinical use.
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