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| Product Name | Quick Exosome Isolation Kit Plus (for Body Fluids) |
| Catalog No. | RESK-0613 |
| Description | Exosomes are small vesicles approximately 30-150 nm in diameter secreted by living cells, with a typical lipid bilayer structure. They are present in cell culture supernatant, serum, plasma, saliva, urine, amniotic fluid, and other biological fluids. Exosomes carry important cargo such as proteins, lipids, DNA, and RNA; they not only play important roles in intercellular material and information transfer, but are also promising early diagnostic biomarkers for a variety of diseases. This kit uses a unique isolation technology to rapidly obtain large amounts of intact exosome particles from body fluids such as cerebrospinal fluid, amniotic fluid, saliva, bronchoalveolar lavage fluid, and nasal lavage fluid. The isolated exosomes are suitable for downstream applications such as cell co-culture, electron microscopy analysis, Western blot, quantitative PCR (qPCR), and high-throughput sequencing. |
| Intended Use | Rapid isolation and purification of intact exosomes from body fluids for downstream research applications. For research use only. |
| Principle / Technology | Precipitation-based exosome enrichment combined with column purification: the exosome concentration solution is mixed with the pre-cleared body fluid and incubated at 4 °C to precipitate exosomes, which are collected by centrifugation, resuspended, and further purified on an EPF purification column (a 220 nm filter column that removes large-particle impurities). |
| Detection Method | Isolated exosomes can be analyzed by electron microscopy, Western blot, qPCR, and high-throughput sequencing. |
| Sample Type | Body fluids such as cerebrospinal fluid, amniotic fluid, saliva, bronchoalveolar lavage fluid, nasal lavage fluid, and ascites (this kit is intended only for body fluid samples; it is not suitable for serum/blood or cell culture supernatant). |
| Performance Range / Specifications | Recommended initial sample volume per extraction: not less than 1 mL for bronchoalveolar lavage fluid, ascites, etc.; 2-3 mL per extraction is recommended. Reagent dosage: 0.25 mL of exosome concentration solution per 1 mL of body fluid; scale proportionally for other sample volumes. |
| Specificity | Designed specifically for exosome isolation from body fluid samples; not applicable to serum/blood or cell culture supernatant. |
| Reaction Conditions / Protocol | 1. Sample pretreatment 1) Collect fresh samples and keep on ice; for frozen samples, thaw in a 25 °C water bath and place on ice once completely melted. 2) Recommended initial sample volume per extraction: not less than 1 mL for bronchoalveolar lavage fluid, ascites, etc.; 2-3 mL per extraction is recommended. 3) Centrifuge at 3,000 x g for 10 min at 4 °C to remove cell debris (if the pellet is large, repeat until no obvious pellet remains, keeping the supernatant each time). 4) Transfer the supernatant to a new tube and centrifuge at 12,000 x g for 10 min at 4 °C to remove impurity debris. 5) Transfer the cleared supernatant to a new centrifuge tube. 2. Exosome extraction 1) Add the exosome concentration solution (Reagent A) to the cleared supernatant: 0.25 mL of reagent per 1 mL of bronchoalveolar lavage fluid or ascites; scale proportionally for other sample volumes. 2) Cap the tube, mix by vortexing for 1 min, then let stand at 4 °C for at least 8 h (longer standing improves yield, but do not exceed 24 h). 3) Centrifuge at 12,000 x g for 30 min at 4 °C and discard the supernatant; the pellet is enriched in exosome particles (aspirate the supernatant as completely as possible). 4) Centrifuge again at 12,000 x g for 2 min at 4 °C and discard the residual supernatant. 5) Resuspend the pellet in an appropriate volume of 1×PBS by gentle pipetting (approximately 100 uL of 1×PBS per 1 mL of body fluid) and transfer to a new 1.5 mL tube. 6) Centrifuge at 12,000 x g for 2 min at 4 °C and keep the supernatant, which is enriched in exosome particles (if the pellet is large, repeat until no obvious pellet remains). 3. Exosome purification 1) Transfer the harvested crude exosome preparation (from the same body fluid source) into the upper chamber of the EPF purification column and centrifuge at 3,000 x g for 10 min at 4 °C; collect the liquid at the bottom of the column tube, which contains the purified exosome particles (the EPF column is single-use and cannot be reused). 2) Aliquot the purified exosomes and store at -80 °C for subsequent experiments. |
| Components / Formulation | Exosome Concentration Solution (nuclease-free, sterile): 6 mL (10 T) / 18 mL (30 T) Exosome Purification Filters (nuclease-free, sterile): 10 tubes (10 T) / 30 tubes (30 T) |
| Storage Conditions | Store at room temperature. |
| Shelf Life | 24 months when stored as recommended. |
| Package Specifications | 10 T 30 T |
| Product Form | Liquid reagent with single-use purification filters. |
| Shipping Conditions | Shipped at room temperature. |
| Expiration Date / Stability | Stable for 24 months at room temperature. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Isolated exosomes are suitable for cell co-culture, electron microscopy analysis, Western blot, qPCR, and high-throughput sequencing. |
| Recommended Buffer System | 1×PBS is recommended for resuspending the exosome pellet. |
| Application Notes / Precautions | Mix the exosome concentration solution thoroughly before use. For further purification, antibody-coated magnetic beads can be used for affinity purification. This kit is intended only for body fluid samples and is not suitable for exosome extraction from serum/blood or cell culture supernatant; please use the cell culture media exosome isolation kit for cell culture supernatant. The purification column is a 220 nm filter column that removes large-particle impurities and cannot be reused. For your safety and health, wear a lab coat and disposable gloves during operation. |
For research use only, not for clinical use.
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