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| Product Name | Quick Exosome Isolation Kit (for Serum/Plasma) |
| Catalog No. | RESK-0608 |
| Description | Exosomes are small vesicles of about 30-150 nm in diameter secreted by living cells, with a typical lipid bilayer structure. They are found in cell culture supernatant, serum, plasma, saliva, urine, amniotic fluid and other biological fluids, and carry important cargos such as proteins, lipids, DNA and RNA. Exosomes play important roles in intercellular material and information transfer and are promising biomarkers for the early diagnosis of many diseases. This kit uses a unique isolation technology to rapidly obtain large quantities of intact exosome particles from serum/plasma, and is suitable for downstream applications such as cell co-culture, electron microscopy, Western blot, quantitative PCR (qPCR) and high-throughput sequencing. |
| Intended Use | Rapid isolation of exosomes from serum or plasma samples for downstream cell co-culture, electron microscopy, Western blot, qPCR and high-throughput sequencing. |
| Principle / Technology | Polymer-based precipitation: the pre-cleared serum/plasma sample is diluted with PBS, mixed with the exosome isolation reagent, incubated at 2-8 °C, and exosomes are pelleted by centrifugation and resuspended in PBS. |
| Detection Method | Isolated exosomes can be characterized by transmission electron microscopy (morphology), nanoparticle tracking analysis (particle size), BCA protein quantification and Western blot. |
| Sample Type | Serum or plasma. Not suitable for other body fluids or cell culture supernatant. |
| Specificity | Specifically designed for exosomes from serum/plasma samples. |
| Reaction Conditions / Protocol | 1) Sample preparation: use fresh samples kept on ice, or thaw frozen samples in a 25 °C water bath and keep on ice after complete thawing. Transfer 1 mL serum/plasma to a centrifuge tube and centrifuge at 3000 x g for 10 min at 4 °C; discard the pellet and transfer the supernatant to a new tube. Centrifuge again at 12,000 x g for 10 min at 4 °C; discard the pellet and transfer the supernatant to a new tube. 2) Add 3 volumes of 1 x PBS to the pre-treated sample and mix thoroughly. 3) Add the corresponding amount of exosome isolation reagent (Component A), cap the tube, vortex for 1 min, and incubate at 2-8 °C for at least 30 min (extending the incubation increases yield, but must not exceed 24 h). 4) Centrifuge at 12,000 x g for 15 min at 4 °C; discard the supernatant and collect the exosome-rich pellet. 5) Centrifuge again at 12,000 x g for 2 min at 4 °C and remove the remaining supernatant. 6) Resuspend the pellet in 1 x PBS by gentle pipetting (recommended initial sample volume to PBS ratio: 1.25:1) and transfer to a new 1.5 mL tube. 7) Centrifuge at 12,000 x g for 2 min at 4 °C; discard the pellet and keep the supernatant, which contains the exosomes. If a visible pellet remains, repeat this washing step. 8) Purified exosomes can be stored at 4 °C for 3 days or aliquoted and stored at -80 °C for long-term storage; avoid repeated freeze-thaw cycles. Optional concentration: use a 3 kDa MWCO ultrafiltration device at 14,000 x g for 10-30 min at 4 °C. Optional sterilization: filter through a 0.22 μm filter before cell co-culture. |
| Components / Formulation | Exosome Isolation Reagent (Component A) |
| Storage Conditions | Store at room temperature. |
| Shelf Life | 24 months at room temperature |
| Package Specifications | 30 mL |
| Product Form | Liquid reagent |
| Shipping Conditions | Shipped at room temperature. |
| Expiration Date / Stability | Stable for 24 months at room temperature. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Isolated exosomes are compatible with cell co-culture, electron microscopy, NTA particle sizing, Western blot, qPCR and high-throughput sequencing. |
| Recommended Buffer System | Dilute samples and resuspend exosomes in 1 x PBS. |
| Application Notes / Precautions | Mix the exosome isolation reagent (Component A) thoroughly before use. Whole blood left standing for a long time, frozen or centrifuged may undergo hemolysis, making exosome extraction impossible; separate serum/plasma properly first. To remove exogenous exosomes from serum used for cell culture, ultracentrifuge the serum at 1 x 10^5 x g for 10 h, or use serum-free medium. This kit is only for serum/plasma samples; for cell culture supernatant exosome isolation, use the dedicated cell culture media kit. For further purification, affinity purification with antibody-coated magnetic beads is recommended. For initial co-culture trials, titrate the exosome protein concentration in the range of 10-100 μg/mL. Wear a lab coat and disposable gloves during operation. |
For research use only, not for clinical use.
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