- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Quick Exosome Isolation Kit (for Cell Culture Media) |
| Catalog No. | RESK-0607 |
| Description | Exosomes are small vesicles of about 30-150 nm in diameter secreted by living cells, with a typical lipid bilayer structure. They are found in cell culture supernatant, serum, plasma, saliva, urine, amniotic fluid and other biological fluids, and carry important cargos such as proteins, lipids, DNA and RNA. Exosomes play important roles in intercellular material and information transfer and are promising biomarkers for the early diagnosis of many diseases. This kit uses a unique isolation technology to rapidly obtain large quantities of intact exosome particles from cell culture supernatant, and is suitable for downstream applications such as cell co-culture, electron microscopy, Western blot, quantitative PCR (qPCR) and high-throughput sequencing. |
| Intended Use | Rapid isolation of exosomes from cell culture supernatant for downstream cell co-culture, electron microscopy, Western blot, qPCR and high-throughput sequencing. |
| Principle / Technology | Polymer-based precipitation: the exosome isolation reagent (a PEG-based precipitation reagent) is mixed with the pre-cleared supernatant; after incubation, exosomes are pelleted by centrifugation and resuspended in PBS. This is a precipitation method, not a magnetic-bead or column-based purification. |
| Detection Method | Isolated exosomes can be characterized by transmission electron microscopy (morphology), nanoparticle tracking analysis (particle size), BCA protein quantification and Western blot. |
| Sample Type | Cell culture supernatant. Not suitable for exosome isolation from serum/plasma. |
| Performance Range / Specifications | Recommended reagent usage (for tumor cells with high exosome secretion): 10 mL supernatant + 2.5 mL isolation reagent for electron microscopy, NTA particle sizing and Western blot; 40 mL supernatant + 10 mL isolation reagent for multi-gene nucleic acid analysis (qPCR) and sequencing. For samples with low exosome secretion (e.g., stem cells), the supernatant volume can be increased proportionally. |
| Specificity | Specifically designed for exosomes from cell culture supernatant. |
| Reaction Conditions / Protocol | 1) Sample preparation: collect cell culture medium, centrifuge at 3000 x g for 10 min, carefully transfer the supernatant to a new tube without disturbing the pellet, and keep on ice. Frozen supernatant (stored at -20 °C or -80 °C) should be thawed in a 25 °C water bath and kept on ice after complete thawing. 2) Mix the exosome isolation reagent thoroughly before use. Add 2.5 mL isolation reagent to 10 mL supernatant, vortex for 1 min, and incubate at 2-8 °C for at least 8 h (extending the incubation increases yield, but must not exceed 24 h). 3) Centrifuge at 10,000 x g for 60 min at 4 °C; discard the supernatant and collect the pellet. 4) Centrifuge again at 10,000 x g for 2 min at 4 °C; discard the remaining supernatant. 5) Resuspend the pellet in 100 μL 1 x PBS by gentle pipetting and transfer to a new 1.5 mL tube. 6) Centrifuge at 12,000 x g for 2 min at 4 °C; discard the pellet and keep the supernatant, which contains the exosomes. 7) Purified exosomes can be stored at 4 °C for 3 days or at -80 °C for long-term storage; avoid repeated freeze-thaw cycles. Optional concentration: use a 3 kDa MWCO ultrafiltration device at 14,000 x g for 10-30 min at 4 °C. Optional sterilization: filter through a 0.22 μm filter before cell co-culture. |
| Components / Formulation | Exosome Isolation Reagent (Component A) |
| Storage Conditions | Store at room temperature. |
| Shelf Life | 24 months at room temperature |
| Package Specifications | 25 mL, 50 mL |
| Product Form | Liquid reagent |
| Shipping Conditions | Shipped at room temperature. |
| Expiration Date / Stability | Stable for 24 months at room temperature. Tighten the cap after use; do not use the reagent if it has dried to a powder. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Isolated exosomes are compatible with cell co-culture, electron microscopy, NTA particle sizing, Western blot, qPCR and high-throughput sequencing. The precipitation method does not affect previously dye-labeled exosomes, and labeled exosomes can be re-extracted with this kit. |
| Recommended Buffer System | Resuspend and wash exosomes in 1 x PBS. |
| Application Notes / Precautions | To ensure that the isolated exosomes originate from your cells, culture cells with exosome-depleted serum; switch to fresh exosome-free or serum-free medium when cells reach 60-70% confluence and collect the supernatant after 24-48 h at about 80-95% confluence. Keep the proportion of dead cells below 5% at harvest, as apoptotic/dead cells release vesicles and impurities that contaminate the exosome preparation. For low-exosome samples (e.g., suspension cells, stem cells, neural cells), no visible pellet may form; wash the inner tube wall with PBS during resuspension and verify the isolate by NTA or BCA assay before downstream experiments. For further purification, affinity purification with antibody-coated magnetic beads is recommended. For initial co-culture trials, titrate the exosome protein concentration in the range of 10-100 μg/mL. This kit is only for cell culture supernatant; for serum/plasma exosome isolation, use the dedicated serum/plasma kit. |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
