- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Quick Exosome Isolation Kit (for Body Fluids) |
| Catalog No. | RESK-0609 |
| Description | Exosomes are small vesicles of about 30-150 nm in diameter secreted by living cells, with a typical lipid bilayer structure. They are found in cell culture supernatant, serum, plasma, saliva, urine, amniotic fluid and other biological fluids, and carry important cargos such as proteins, lipids, DNA and RNA. Exosomes play important roles in intercellular material and information transfer and are promising biomarkers for the early diagnosis of many diseases. This kit uses a unique isolation technology to rapidly obtain large quantities of intact exosome particles from body fluids such as cerebrospinal fluid, amniotic fluid, saliva, bronchoalveolar lavage fluid and nasal lavage fluid, and is suitable for downstream applications such as cell co-culture, electron microscopy, Western blot, quantitative PCR (qPCR) and high-throughput sequencing. |
| Intended Use | Rapid isolation of exosomes from body fluid samples for downstream cell co-culture, electron microscopy, Western blot, qPCR and high-throughput sequencing. |
| Principle / Technology | Polymer-based precipitation: the pre-cleared body fluid sample is mixed with the exosome isolation solution, incubated at 2-8 °C, and exosomes are pelleted by centrifugation and resuspended in PBS. |
| Detection Method | Isolated exosomes can be characterized by transmission electron microscopy (morphology), nanoparticle tracking analysis (particle size), BCA protein quantification and Western blot. |
| Sample Type | Body fluids such as cerebrospinal fluid, amniotic fluid, saliva, bronchoalveolar lavage fluid and nasal lavage fluid (minimum 1 mL per sample). Not suitable for serum/plasma or cell culture supernatant. |
| Specificity | Specifically designed for exosomes from body fluid samples. |
| Reaction Conditions / Protocol | 1) Sample preparation: use fresh samples kept on ice, or thaw frozen samples in a 25 °C water bath and keep on ice after complete thawing. Transfer 1 mL (not less than 1 mL) of body fluid to a centrifuge tube and centrifuge at 3000 x g for 10 min at 4 °C; discard the pellet and transfer the supernatant to a new tube. Centrifuge again at 12,000 x g for 10 min at 4 °C; discard the pellet and transfer the supernatant to a new tube. 2) Add the exosome isolation solution to the pre-treated sample according to the reagent-to-sample ratio; scale proportionally for other sample volumes. Cap the tube, vortex for 1 min, and incubate at 2-8 °C for at least 8 h (extending the incubation increases yield, but must not exceed 24 h). 3) Centrifuge at 12,000 x g for 30 min at 4 °C; discard the supernatant and collect the exosome-rich pellet. 4) Centrifuge again at 12,000 x g for 2 min at 4 °C and remove the remaining supernatant. 5) Resuspend the pellet in 1 x PBS by gentle pipetting (recommended initial sample volume to PBS ratio: 10:1) and transfer to a new 1.5 mL tube. 6) Centrifuge at 12,000 x g for 2 min at 4 °C; discard the pellet and keep the supernatant, which contains the exosomes. If a visible pellet remains, repeat this washing step. 7) Purified exosomes can be stored at 4 °C for 3 days or aliquoted and stored at -80 °C for long-term storage; avoid repeated freeze-thaw cycles. Optional concentration: because body fluids contain abundant contaminating proteins, further purification with a 100 kDa MWCO ultrafiltration device (14,000 x g for 10-30 min at 4 °C) or antibody-coated magnetic beads is recommended before cell co-culture or high-throughput sequencing. Optional sterilization: filter through a 0.22 μm filter before cell co-culture. |
| Components / Formulation | Exosome Isolation Solution |
| Storage Conditions | Store at room temperature. |
| Shelf Life | 24 months at room temperature |
| Package Specifications | 10 T (5 mL), 30 T (15 mL) |
| Product Form | Liquid reagent |
| Shipping Conditions | Shipped at room temperature. |
| Expiration Date / Stability | Stable for 24 months at room temperature. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Isolated exosomes are compatible with cell co-culture, electron microscopy, NTA particle sizing, Western blot, qPCR and high-throughput sequencing. |
| Recommended Buffer System | Resuspend and wash exosomes in 1 x PBS. |
| Application Notes / Precautions | Mix the exosome isolation reagent (Component A) thoroughly before use. This kit is only for body fluid samples; for cell culture supernatant exosome isolation, use the dedicated cell culture media kit. For further purification, affinity purification with antibody-coated magnetic beads is recommended. For initial co-culture trials, titrate the exosome protein concentration in the range of 10-100 μg/mL. Wear a lab coat and disposable gloves during operation. |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
