| Product Name | Pyruvate Kinase (PK) Assay Reagent (Colorimetric Method) |
| Catalog No. | ETR-HMM-0056 |
| Description | Pyruvate kinase is one of the three rate-limiting enzymes in the anaerobic glycolysis pathway of red blood cells, directly affecting red blood cell energy metabolism. Deficiency of this enzyme is an autosomal recessive genetic disorder that may lead to non-spherocytic hemolytic anemia. The function of pyruvate kinase is to catalyze the direct glycolytic process, transferring the phosphate group from phosphoenolpyruvate to adenosine diphosphate (ADP), thereby phosphorylating it into high-energy adenosine triphosphate (ATP). Pyruvate kinase deficiency reduces ATP production, thereby impairing red blood cell membrane function, shortening red blood cell survival time, and causing hemolytic anemia. Additionally, serum pyruvate kinase levels have significant scientific significance in assessing myocardial injury. |
| Application | This product uses the colorimetric method to measure PK activity. |
| Applicable Instruments | UV Spectrophotometer |
| Number of Testable Samples | 48 Samples |
| Detection Time | 30 min (20-40 Samples) |
| Detection Method | Colorimetric method |
| Spectral Parameters | 370 nm |
| Standard Linear Range | 1.3-250 U/L |
| Detection Limit | 1.3 U/L |
| Inter-batch Variation CV % | 6.55 |
| Intra-batch Variation CV % | 3.4 |
| Coefficient of Variation CV % | 1.7 |
| Recovery Rate % | 101 |
| Storage | 2-8°C. Reagents R2, R4 and R5 must be stored at -20°C. |
| Shelf Life | 3 months |
For research use only, not for clinical use.
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