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| Product Name | Pyruvate Assay Kit (Fluorometric) |
| Catalog No. | CMTR-HMM-0069 |
| Description | A fluorometric assay kit for the quantitative determination of pyruvate in biological samples. The assay involves enzymatic oxidation of pyruvate by pyruvate oxidase, generating hydrogen peroxide that reacts with a fluorogenic probe in the presence of horseradish peroxidase to produce a fluorescent signal. The high sensitivity of fluorometric detection makes this kit suitable for samples with limited pyruvate content. |
| Intended Use | Sensitive quantification of pyruvate levels in cell culture media, cell lysates, and biological fluids for metabolic flux analysis. |
| Principle / Technology | Pyruvate oxidase enzymatic conversion coupled with fluorogenic peroxidase detection |
| Detection Method | Fluorescence (excitation 535 nm, emission 587 nm) |
| Sample Type | Cell culture medium, cell and tissue lysates, serum, plasma, urine |
| Performance Range / Specifications | Linear range: 0.2–50 µM pyruvate in reaction well |
| Sensitivity / LOD | Detection limit: 0.1 µM |
| Specificity | Pyruvate oxidase specific to pyruvate; no cross-reactivity with lactate, acetate, or other monocarboxylates |
| Reaction Conditions / Protocol | Deproteinize samples if needed; add sample to reaction mix; incubate 30 min at room temperature protected from light; read fluorescence |
| Components / Formulation | Pyruvate oxidase enzyme mix, fluorogenic probe, HRP, pyruvate standard (10 mM), assay buffer |
| Storage Conditions | –20 °C for enzymes and probe; 2–8 °C for buffer and standard |
| Shelf Life | 6 months at –20 °C |
| Package Specifications | 100 tests |
| Product Form | Liquid reagents; lyophilized probe and enzyme |
| Quality Control | Standard concentration verified by enzymatic endpoint assay; intra-assay CV ≤8% |
| Key Features | Fluorometric sensitivity approximately 10× higher than colorimetric pyruvate assays |
| Purity | Reagent grade; fluorescent probes ≥95% pure by HPLC |
| Concentration | As specified per kit format; probe stock solutions in DMSO as specified |
| Activity / Unit Definition | Standard curve linearity R² ≥0.99 per lot; dynamic range as specified |
| Molecular Weight | Varies by probe and buffer component |
| Source / Origin | Synthetic fluorescent probes; recombinant enzymes; purified metabolite standards |
| pH Range / Optimal pH | pH 7.2–7.4 for cell-based assays; pH varies for biochemical metabolite detection |
| Shipping Conditions | Cold packs or dry ice for probes and enzymes; protect from light |
| Expiration Date / Stability | 6–12 months at recommended storage temperature |
| Regulatory / Compliance | Research use; manufactured under ISO 9001 |
| Compatibility | Compatible with fluorescence microplate readers, luminometers, and spectrophotometers |
| Recommended Buffer System | Assay-specific buffer optimized per product protocol |
| Application Notes / Precautions | Normalize signal to cell number or protein content; avoid probe photobleaching; include appropriate antioxidant controls for ROS assays |
| Batch-to-Batch Consistency | Standard curve parameters within ±15% of reference lot |
For research use only, not for clinical use.
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