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| Product Name | Proteinase K Digestion Solution, 20 mg/mL, Molecular Biology Grade |
| Catalog No. | ATR-SPS-0001 |
| Description | A highly concentrated solution of recombinant Proteinase K in a glycerol-stabilized storage buffer, free of DNase and RNase activity, for the enzymatic digestion of proteins during nucleic acid purification. |
| Intended Use | Digestion of contaminating proteins in DNA and RNA extraction protocols; inactivation of nucleases during nucleic acid purification; tissue dissociation for genomic DNA extraction. |
| Principle / Technology | Proteinase K is a broad-specificity serine protease that cleaves peptide bonds adjacent to aliphatic and aromatic amino acid residues; it remains active in the presence of SDS, urea, and EDTA, making it ideal for use with denaturing lysis buffers. |
| Detection Method | Protein digestion monitored by SDS-PAGE, nuclease inactivation verified by nucleic acid stability assay |
| Sample Type | Mammalian tissues, cultured cells, bacterial pellets, yeast, body fluids |
| Performance Range / Specifications | Activity ≥30 U/mg protein; effective in 0.5% SDS, 10 mM EDTA, 4 M urea |
| Sensitivity / LOD | N/A |
| Specificity | Broad spectrum: cleaves keratin, collagen, albumin, histones, and nucleases |
| Reaction Conditions / Protocol | Use at 100–200 µg/mL final concentration; incubate at 56°C for 30 min to 2 hours; heat inactivate at 95°C for 10 min if required. |
| Components / Formulation | Recombinant Proteinase A in 50 mM Tris-HCl pH 8.0, 50% glycerol, 5 mM calcium chloride |
| Storage Conditions | -20°C; avoid repeated freeze-thaw cycles |
| Shelf Life | 24 months at -20°C; 12 months at 2–8°C |
| Package Specifications | 100 mg (5 mL), 1 g |
| Product Form | Liquid enzyme solution |
| Quality Control | DNase and RNase free, specific activity by hemoglobin digestion assay, purity by SDS-PAGE |
| Key Features | Recombinant source ensures nuclease free; high specific activity; active in SDS and urea; glycerol stabilized; standard for DNA extraction |
For research use only, not for clinical use.
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