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| Product Name | Protein Precipitation Reagent for Biological Fluids (Acetonitrile-Based) |
| Catalog No. | ATR-SPS-0020 |
| Description | A ready-to-use acetonitrile-based protein precipitation reagent for the rapid removal of proteins from serum, plasma, and other biological fluids prior to small molecule analysis. The organic solvent denatures and precipitates proteins within seconds, while the supernatant containing the deproteinized sample is suitable for direct injection into LC-MS or HPLC systems. This preparation method is the most widely used sample cleanup step for therapeutic drug monitoring and metabolomics workflows. |
| Intended Use | Rapid depletion of proteins from biological fluid samples for chromatographic and mass spectrometric analysis. |
| Principle / Technology | Organic solvent-mediated protein denaturation and precipitation |
| Detection Method | LC-MS/MS, HPLC-UV, or HPLC-FLD of small molecule analytes in supernatant |
| Sample Type | Human serum, plasma, urine, cerebrospinal fluid, and cell culture medium |
| Performance Range / Specifications | Protein removal efficiency: ≥99.5%; typical supernatant volume recovery: ≥90% of total liquid volume |
| Sensitivity / LOD | Small molecule recovery: 85–110% for compounds spanning LogP –2 to +6 |
| Specificity | Precipitates all protein classes non-selectively; small molecules including drugs, metabolites, and endogenous compounds remain in supernatant |
| Reaction Conditions / Protocol | Add 3 volumes of precipitation reagent to 1 volume sample; vortex 30 sec; centrifuge 10 min at 14,000 × g; collect supernatant for analysis |
| Components / Formulation | Acetonitrile with 0.1% formic acid and internal standard placeholder (user adds specific internal standard per application) |
| Storage Conditions | Room temperature; flammable liquid — store away from ignition sources |
| Shelf Life | 24 months in sealed container |
| Package Specifications | 500 mL, 1 L |
| Product Form | Liquid, clear, organic odor |
| Quality Control | Small molecule analyte recovery verified with a panel of 20 therapeutic drugs across diverse chemical classes |
| Key Features | Single-step protein crash with acidified acetonitrile achieves cleaner chromatographic baselines than methanol precipitation for most analytes |
| Purity | DNase/RNase free; sterile-filtered where applicable |
| Concentration | As specified on product label |
| Activity / Unit Definition | Sample processing efficiency ≥90% recovery per validation protocol |
| Molecular Weight | Not applicable or as specified for enzyme components |
| Source / Origin | Synthetic and recombinant components as specified |
| pH Range / Optimal pH | As stated in product specification |
| Shipping Conditions | Ambient for buffers; cold packs for enzyme components |
| Expiration Date / Stability | 12–18 months at recommended storage temperature |
| Regulatory / Compliance | Research use; manufactured under ISO 9001 or ISO 13485 quality system |
| Compatibility | Compatible with downstream PCR, mass spectrometry, and immunoassay workflows |
| Recommended Buffer System | Use as directed; components optimized for stated application |
| Application Notes / Precautions | Work quickly to prevent sample degradation; use appropriate protease/phosphatase inhibitors if needed |
| Batch-to-Batch Consistency | Protein precipitation efficiency and analyte recovery within ±15% of reference lot |
For research use only, not for clinical use.
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