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| Product Name | Protease Inhibitor Cocktail for Plant Cell and Tissue Extracts, EDTA-Free, 100x DMSO Stock |
| Catalog No. | RERE-0044 |
| Description | Plant extracts carry a distinctive protease spectrum, so this cocktail pairs AEBSF, bestatin, E-64, leupeptin, pepstatin A and 1,10-phenanthroline to cover cysteine, serine and aspartyl proteases, metalloproteases and aminopeptidases in one addition. Supplied as a 100x DMSO stock, it doses at 1:100 into extraction buffers for plant cell and tissue work, with downstream applications spanning Western blotting, co-immunoprecipitation, pull-down assays, immunofluorescence, immunohistochemistry and kinase research. |
| Intended Use | Protease suppression during plant protein extraction for Western blotting, Co-IP, pull-down, IF, IHC and kinase research |
| Principle / Technology | Broad-spectrum inhibition of cysteine, serine, aspartyl proteases, metalloproteases and aminopeptidases from a single DMSO stock |
| Detection Method | Cysteine, serine, aspartyl and metalloproteases plus aminopeptidases of plant origin |
| Sample Type | Plant cell and tissue extracts |
| Performance Range / Specifications | 100x dosing with five-inhibitor coverage of the plant protease spectrum |
| Specificity | Broad protease-class coverage tuned to plant material, EDTA-free |
| Reaction Conditions / Protocol | Thaw, mix and dilute 1:100 into the plant extraction buffer before grinding or lysis |
| Components / Formulation | 1 mL of 100x DMSO stock per vial |
| Storage Conditions | -25 to -15°C for 12 months |
| Shelf Life | 12 months |
| Package Specifications | 1 mL |
| Product Form | DMSO solution, 100x concentrate |
| Quality Control | Inhibitory spectrum verified on each lot |
| Key Features | Tuned to plant protease profiles; covers aminopeptidase activity often missed elsewhere; EDTA-free; 100x DMSO format |
| Concentration | 100x stock in DMSO |
| Activity / Unit Definition | Broad-spectrum protease and aminopeptidase inhibition |
| Source / Origin | Formulated blend of defined inhibitor compounds |
| Shipping Conditions | Wet ice or frozen packs |
| Expiration Date / Stability | 12 months at -25 to -15°C |
| Regulatory / Compliance | For research use only |
| Compatibility | Suited to plant extraction feeding into Western blotting, Co-IP, pull-down assays, IF, IHC and kinase research |
| Recommended Buffer System | AEBSF, bestatin, E-64, leupeptin, pepstatin A and 1,10-phenanthroline in DMSO |
| Application Notes / Precautions | DMSO carries dissolved compounds through skin; wear gloves; aliquot to avoid repeated freeze-thaw |
| Batch-to-Batch Consistency | Lot-numbered packaging; lot record retained for traceability |
| Starting Material / Tissue Site | Plant cell or tissue lysates supplied by the user |
| Delivery Standard | Standard catalogue shipment, packaging integrity verified before dispatch |
| Readouts / Downstream Assays | Western blotting, Co-IP, pull-down assays, immunofluorescence, immunohistochemistry, kinase research |
For research use only, not for clinical use.
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