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| Product Name | Protease Inhibitor Cocktail for His-Tagged Protein Purification, EDTA-Free, 100x DMSO Stock |
| Catalog No. | RERE-0043 |
| Description | Purifying His-tagged proteins on metal-chelate media demands an inhibitor blend that protects the target without stripping nickel from the column, which is exactly what this EDTA-free cocktail delivers. The 100x DMSO stock combines AEBSF, bestatin, E-64, pepstatin A and the disodium salt of phosphoramidon, covering cysteine, serine and aspartyl proteases together with thermolysin and aminopeptidase activity. One addition at 1:100 protects His-tag purification runs as well as Western blotting, Co-IP, pull-down, IF, IHC and kinase assays. |
| Intended Use | Protease protection during His-tagged protein purification and in Western blotting, Co-IP, pull-down, IF, IHC and kinase research |
| Principle / Technology | Broad protease-class inhibition without EDTA, preserving immobilised metal affinity chromatography performance |
| Detection Method | Cysteine, serine and aspartyl proteases, thermolysin and aminopeptidases |
| Sample Type | Lysates destined for His-tag purification and immunoassay workflows |
| Performance Range / Specifications | 100x dosing; chelator-free so nickel column capacity is preserved |
| Specificity | Broad protease-class coverage with EDTA deliberately omitted for metal-chelate purification |
| Reaction Conditions / Protocol | Thaw, mix and dilute 1:100 into the lysis or column-loading buffer |
| Components / Formulation | 1 mL of 100x DMSO stock per vial |
| Storage Conditions | -25 to -15°C for 12 months |
| Shelf Life | 12 months |
| Package Specifications | 1 mL |
| Product Form | DMSO solution, 100x concentrate |
| Quality Control | Inhibitory spectrum verified on each lot |
| Key Features | Designed around His-tag purification constraints; EDTA-free; covers thermolysin and aminopeptidase activity; convenient 100x DMSO format |
| Concentration | 100x stock in DMSO |
| Activity / Unit Definition | Broad-spectrum protease inhibition without chelation |
| Source / Origin | Formulated blend of defined inhibitor compounds |
| Shipping Conditions | Wet ice or frozen packs |
| Expiration Date / Stability | 12 months at -25 to -15°C |
| Regulatory / Compliance | For research use only |
| Compatibility | Explicitly compatible with immobilised metal affinity purification of His-tagged proteins and downstream immunoassays |
| Recommended Buffer System | AEBSF, bestatin, E-64, pepstatin A and phosphoramidon disodium salt in DMSO |
| Application Notes / Precautions | DMSO carries dissolved compounds through skin; wear gloves; aliquot to avoid repeated freeze-thaw |
| Batch-to-Batch Consistency | Lot-numbered packaging; lot record retained for traceability |
| Starting Material / Tissue Site | His-tag expressing lysates supplied by the user |
| Delivery Standard | Standard catalogue shipment, packaging integrity verified before dispatch |
| Readouts / Downstream Assays | His-tagged protein purification, Western blotting, Co-IP, pull-down assays, IF, IHC, kinase research |
For research use only, not for clinical use.
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