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| Product Name | Protease Inhibitor Cocktail for Fungal and Yeast Extracts, EDTA-Free, 100x DMSO Stock |
| Catalog No. | RERE-0042 |
| Description | Fungal and yeast lysates release particularly aggressive endogenous proteases, so extraction needs a broad and robust inhibitor panel. This concentrated cocktail dissolves in DMSO as a 100x stock and combines AEBSF, E-64, pepstatin A and 1,10-phenanthroline to cover serine, cysteine, aspartyl and metalloproteases in one addition. The EDTA-free formulation keeps metal-dependent downstream steps intact, and the 100x format minimises the volume added to each extraction. Typical applications span Western blotting, co-immunoprecipitation, pull-down assays, immunofluorescence, immunohistochemistry and kinase research. |
| Intended Use | Protease suppression during fungal and yeast protein extraction for Western blotting, Co-IP, pull-down, IF, IHC and kinase research |
| Principle / Technology | Broad-spectrum inhibition of serine, cysteine, aspartyl and metalloprotease classes from a single DMSO stock addition |
| Detection Method | Serine, cysteine, aspartyl and metalloproteases of fungal and yeast origin |
| Sample Type | Fungal and yeast crude extracts and cell lysates |
| Performance Range / Specifications | 100x dosing with one-dilution convenience; broad four-class protease coverage |
| Specificity | Broad protease-class coverage without EDTA chelation |
| Reaction Conditions / Protocol | Thaw, mix and dilute 1:100 into the extraction buffer before lysis |
| Components / Formulation | 1 mL of 100x DMSO stock per vial |
| Storage Conditions | -25 to -15°C for 12 months |
| Shelf Life | 12 months |
| Package Specifications | 1 mL |
| Product Form | DMSO solution, 100x concentrate |
| Quality Control | Inhibitory spectrum verified on each lot |
| Key Features | Tuned to fungal and yeast protease profiles; EDTA-free; 100x DMSO format saves handling; four protease classes covered at once |
| Concentration | 100x stock in DMSO |
| Activity / Unit Definition | Broad-spectrum protease inhibition |
| Source / Origin | Formulated blend of defined inhibitor compounds |
| Shipping Conditions | Wet ice or frozen packs |
| Expiration Date / Stability | 12 months at -25 to -15°C |
| Regulatory / Compliance | For research use only |
| Compatibility | Suited to Western blotting, Co-IP, pull-down assays, IF, IHC and kinase research on fungal and yeast extracts |
| Recommended Buffer System | AEBSF, E-64, pepstatin A and 1,10-phenanthroline in DMSO |
| Application Notes / Precautions | DMSO carries dissolved compounds through skin; wear gloves; avoid repeated freeze-thaw by aliquoting |
| Batch-to-Batch Consistency | Lot-numbered packaging; lot record retained for traceability |
| Starting Material / Tissue Site | Fungal or yeast lysates supplied by the user |
| Delivery Standard | Standard catalogue shipment, packaging integrity verified before dispatch |
| Readouts / Downstream Assays | Western blotting, Co-IP, pull-down assays, immunofluorescence, immunohistochemistry, kinase research |
For research use only, not for clinical use.
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