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| Product Name | Protease Inhibitor Cocktail, EDTA-Free, Mini Tablets |
| Catalog No. | RERE-0037 |
| Description | Crude protein extracts released by cell lysis carry a heavy load of endogenous proteases, so an inhibitor blend is needed to keep target proteins intact through purification and analysis. These mini tablets each dissolve within about one minute in 10 mL of cell or tissue lysate and deliver a broad-spectrum blend of AEBSF, aprotinin, bestatin, E-64, leupeptin and pepstatin A. The formulation omits EDTA, making the tablets suitable for immobilised metal ion affinity chromatography, two-dimensional gel electrophoresis and native gel workflows where chelators would interfere. |
| Intended Use | Protease suppression during extraction from animal, plant, bacterial and yeast samples; protection in IMAC purification, 2D electrophoresis and native gel analysis |
| Principle / Technology | Broad-spectrum inhibition of serine, cysteine, aspartyl proteases and aminopeptidases without metal chelation |
| Detection Method | Endogenous serine proteases, cysteine proteases, aminopeptidases and acidic proteases |
| Sample Type | Lysates of animal, plant, bacterial or yeast origin |
| Performance Range / Specifications | One tablet protects 10 mL of lysate; dissolved tablets keep the solution protected for at least 2 weeks at 4°C |
| Specificity | Broad protease coverage with EDTA omitted to preserve metal-dependent purification and analysis steps |
| Reaction Conditions / Protocol | Drop one mini tablet into 10 mL of lysis solution, allow about 1 minute to dissolve, then vortex to homogeneity |
| Components / Formulation | Mini protease inhibitor tablets, 10 or 50 tablets per glass vial |
| Storage Conditions | 2 to 8°C for at least 12 months as supplied |
| Shelf Life | At least 12 months |
| Package Specifications | 1 vial of 10 tablets; 1 vial of 50 tablets |
| Product Form | Mini tablets in a glass vial |
| Quality Control | Dissolution time and inhibitory spectrum verified on each lot |
| Key Features | Dissolves in about one minute; EDTA-free for IMAC and 2D-PAGE compatibility; broad species coverage; room-temperature shipping |
| Concentration | One tablet per 10 mL of lysate |
| Activity / Unit Definition | Broad-spectrum protease inhibition without chelation |
| Source / Origin | Formulated blend of defined inhibitor compounds |
| Shipping Conditions | Ambient temperature |
| Expiration Date / Stability | At least 12 months at 2 to 8°C; dissolved inhibitor solution stable at least 2 weeks at 4°C |
| Regulatory / Compliance | For research use only |
| Compatibility | Explicitly suited to immobilised metal ion affinity chromatography, 2D gel electrophoresis and native PAGE |
| Recommended Buffer System | Tableted blend of AEBSF, aprotinin, bestatin, E-64, leupeptin and pepstatin A |
| Application Notes / Precautions | Handle tablets with dry gloves; confirm complete dissolution before starting the extraction |
| Batch-to-Batch Consistency | Lot-numbered packaging; lot record retained for traceability |
| Starting Material / Tissue Site | Animal, plant, bacterial or yeast lysates supplied by the user |
| Delivery Standard | Standard catalogue shipment, packaging integrity verified before dispatch |
| Readouts / Downstream Assays | Protein extraction and purification feeding into Western blotting, IMAC, 2D electrophoresis and native gels |
For research use only, not for clinical use.
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