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| Product Name | Proline (Pro) Content Assay Kit, 100T/96S, 200T/192S |
| Catalog No. | AACAK-YJL-0016 |
| Detection Method | Micro-volume method |
| Storage | Store at 2-8°C, 6 months |
| Intended Use | For research use only. |
| Note | For your safety and health, please wear lab coat, disposable gloves and mask during operation. |
Proline is a non-essential amino acid with a unique cyclic structure that plays pivotal roles in various biological processes. It is widely recognized for its contributions to protein structure stabilization, as its cyclic side chain imposes specific conformational constraints on peptides and proteins, influencing their folding and functional properties.
In research settings, proline content analysis is essential across multiple fields. In plant biology, it serves as a key indicator of stress tolerance—plants accumulate proline in response to drought, salinity, cold, or heavy metal stress to maintain cellular osmotic balance and protect biomolecules. In animal and medical research, proline metabolism is linked to collagen synthesis, wound healing, and pathological conditions such as fibrosis and metabolic disorders.
Accurate and reliable quantification of proline is critical for advancing research in these areas. Traditional detection methods often involve complex sample preparation, long reaction times, or low sensitivity. The Proline (Pro) Content Assay Kit is developed to address these challenges, providing a streamlined solution for researchers to measure proline levels efficiently in various sample types.
Adopts the micro-volume method, enabling high-throughput analysis with minimal sample consumption (suitable for small-volume samples like tissue homogenates, cell lysates, or plant extracts).
Offers a linear detection range optimized for common research samples, ensuring accurate quantification of proline concentrations within biologically relevant levels.
Requires simple and straightforward operating procedures, reducing experimental complexity and minimizing the risk of human error.
Stable reagent performance under recommended storage conditions (2-8°C), with a 6-month shelf life that guarantees consistent results over an extended period.
Compatible with standard microplate readers, eliminating the need for specialized equipment and facilitating integration into existing laboratory workflows.
High sensitivity: Can detect low concentrations of proline, ensuring reliable results even for samples with trace amounts of the target analyte.
Excellent specificity: Minimizes interference from other amino acids and biological matrix components, ensuring accurate proline quantification without cross-reactivity.
Time-efficient: Shortens the overall experimental cycle compared to traditional methods, allowing researchers to complete more assays in less time.
Cost-effective: Provides sufficient reagents for 100 or 200 tests (corresponding to 96 or 192 well formats), offering competitive value for high-throughput research needs.
Research-focused design: Tailored to meet the rigorous demands of academic and industrial research, with results that support data reproducibility and publication-quality outcomes.
For research use only, not for clinical use.
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