- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Human Homocysteine (HCY) ELISA Kit |
| Catalog No. | DB-2534 |
| Description | Oxidized Hcy is converted into free Hcy, which, in an enzyme-catalyzed cyclic reaction, converts NADH into NAD+, causing a decrease in the optical density (OD) at 340 nm. The Hcy concentration in the sample is calculated based on the rate of this decrease. |
| Kit Components | Reagent 1: Buffer solution A, 10 mL×1 bottle Reagent 2: Buffer solution B, 6 mL×1 bottle Reagent 3: Substrate, 1 powder agent Reagent 4: Enzyme reagent, 2 powder agents Reagent 5: 28 μmol/L standard, 0.5 mL×1 piece UV enzyme standard plate: 1 piece with 96 pores Sample position marking table: 1 piece |
| Materials Required But Not Provided | Equipment: Microplate reader (330–350 nm, optimal detection wavelength 340 nm) |
| Reagent Preparation | Before testing, allow all reagents to equilibrate to room temperature. Preparation of Reagent 3 Working Solution: Add 1 mL of Reagent 1 to one vial of Reagent 3 and mix thoroughly. After aliquoting, store the prepared Reagent 3 Working Solution at -20°C away from light for up to 5 days. Avoid repeated freeze-thaw cycles. Preparation of Reagent 4 Working Solution: Add 200 μL of double-distilled water to one vial of Reagent 4 to dissolve it. Store the prepared Reagent 4 Working Solution in the dark at 2–8°C for up to 1 day. Preparation of Reaction Working Solution A: Mix Reagent 1 and Reagent 3 working solution in a 15:1 volume ratio and mix thoroughly. Store the prepared Reaction Working Solution A at 2–8°C, protected from light; it is valid for use within 1 day. Preparation of Reaction Working Solution B: Mix Reagent 2 and Reagent 4 working solution in an 11:1 volume ratio and mix thoroughly. Store the prepared Reaction Working Solution B at 2–8°C, protected from light; it is valid for use within 1 day. |
| Sample Preparation | Serum and urine samples can be tested directly. Sample Dilution: Prior to formal testing, select 2–3 samples expected to show significant variation and dilute them to different concentrations for preliminary testing. |
| Test Procedure | Standard wells: Add 20 μL of Reagent 5 to each standard well. Blank wells: Add 20 μL of double-distilled water to each blank well. Test wells: Add 20 μL of the sample to be tested to the corresponding test wells. Add 120 μL of Reaction Buffer A to each well from Step 1, shake the plate for 3 seconds to mix, and incubate at 37°C for 5 minutes; Add 80 μL of Reaction Buffer B to each well from Step 2, shake the plate for 3 seconds to mix, and incubate at 37°C for 2 minutes. Measure the OD value A1 at a wavelength of 340 nm using a microplate reader. After incubating at 37°C for 10 minutes, measure the OD value A2, and calculate ∆A = A1 – A2. |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.