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| Product Name | Glutamic Acid (Glu) Content Assay Kit, 50T/48S |
| Catalog No. | AACAK-YJL-0023 |
| Detection Method | UV spectrophotometry, Spectrophotometer |
| Storage | Store at -20°C, 6 months |
| Intended Use | For research use only. |
| Note | For your safety and health, please wear lab coat, disposable gloves and mask during operation. |
Glutamic acid (Glu) is one of the most abundant non-essential amino acids in living organisms, playing pivotal roles in multiple biological processes that underpin both basic research and applied scientific investigations. It serves as a key neurotransmitter in the central nervous system, mediating excitatory synaptic transmission and influencing cognitive functions such as learning, memory, and neural plasticity. Beyond its neurobiological significance, Glu is a critical intermediate in cellular metabolism—participating in the tricarboxylic acid (TCA) cycle, nitrogen metabolism, and the synthesis of other amino acids, peptides, and bioactive molecules.
In research settings, accurate quantification of Glu content is essential across diverse fields:
Neurobiology and neuroscience: Studying synaptic function, neurodegenerative diseases (e.g., Alzheimer’s, Parkinson’s), and neurodevelopmental disorders.
Biochemistry and molecular biology: Investigating metabolic pathways, enzyme activity, and cellular stress responses.
Agriculture and food science: Evaluating nutritional value of crops, feed ingredients, and fermented products, as well as monitoring flavor development in food processing.
Pharmacology and toxicology: Assessing drug efficacy, toxicity mechanisms, and the impact of xenobiotics on cellular metabolism.
Traditional methods for Glu detection, such as high-performance liquid chromatography (HPLC) and capillary electrophoresis, often require complex sample pretreatment, expensive instrumentation, and lengthy analysis times. The demand for a rapid, sensitive, and user-friendly assay kit has grown exponentially to meet the needs of high-throughput research and routine laboratory testing. Our Glutamic Acid (Glu) Content Assay Kit is developed to address these challenges, providing a reliable solution for precise Glu quantification in various sample types.
Utilizes UV spectrophotometry, a well-established and widely accessible detection technique that ensures high reproducibility and accuracy.
Compatible with multiple sample matrices, including cell lysates, tissue homogenates, serum, plasma, urine, plant extracts, and food samples.
Requires minimal sample volume (typically 10-50 μL per assay), preserving valuable samples for further analysis.
Simple and streamlined experimental procedure with clear step-by-step instructions, reducing hands-on time to less than 2 hours from sample preparation to results.
Stable reagents with a 6-month storage life at -20°C, ensuring consistent performance and reducing the need for frequent reagent replacement.
Linear detection range optimized to cover physiological and experimental Glu concentrations, avoiding the need for excessive sample dilution or concentration.
Low interference from common coexisting substances (e.g., other amino acids, carbohydrates, lipids), ensuring specific detection of Glu.
High sensitivity with a detection limit as low as 0.1 μmol/L, enabling the quantification of low-abundance Glu in dilute samples.
Excellent precision with intra-assay and inter-assay CV (coefficient of variation) below 5%, ensuring reliable and consistent results across experiments.
Cost-effective compared to advanced techniques like HPLC or mass spectrometry, eliminating the need for specialized equipment or trained operators.
Ready-to-use reagent components, pre-mixed and pre-aliquoted to minimize preparation errors and save laboratory time.
Versatile for both small-scale experiments and high-throughput screening, adapting to the needs of individual researchers and large research teams.
Validated through extensive testing with standard reference materials and real sample matrices, ensuring compliance with research quality standards.
Comprehensive technical support, including troubleshooting guidance and application notes, to assist users in achieving optimal results.
For research use only, not for clinical use.
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