Amino Acid (AA) Content Assay Kit, 50T/48S

Amino acids are fundamental biomolecules that play irreplaceable roles in both biological systems and scientific research. They serve as the building blocks of proteins, which are essential for the structure, function, and regulation of cells, tissues, and organs across all living organisms. Beyond protein synthesis, amino acids also act as key intermediates in numerous metabolic pathways, participate in neurotransmitter production, and contribute to the maintenance of cellular redox balance—making their quantitative analysis critical for understanding biological processes at the molecular level.

In research settings, the accurate measurement of amino acid content provides valuable insights into multiple fields:

Animal Physiology and Pathology: Amino acids are primarily metabolized in the liver and kidneys of animals. Changes in the amino acid profile of biological samples (such as urine, serum, or plasma) can directly reflect the physiological state of these vital organs. For example, abnormal amino acid levels may indicate liver dysfunction, kidney impairment, or responses to stressors like burns or infectious diseases (e.g., typhoid). This makes amino acid analysis a powerful tool for studying animal health, disease progression, and the efficacy of therapeutic interventions.
Plant Biology and Agriculture: In plants, amino acid content is closely linked to nitrogen metabolism—a core process that governs plant growth, development, and stress resistance. Measuring amino acids in plant tissues (e.g., leaves, roots, or seeds) allows researchers to evaluate how plants absorb, transport, and assimilate nitrogen under different environmental conditions (such as varying soil nutrient levels, drought, or temperature stress) and at different growth stages (e.g., seedling, flowering, or fruiting). This data is essential for optimizing agricultural practices, developing stress-tolerant crop varieties, and improving crop yield and nutritional quality.
Cellular and Molecular Research: In cell culture models or microbial studies, amino acid quantification helps monitor cellular metabolism, nutrient utilization, and the impact of experimental treatments (e.g., drug exposure, gene editing) on cellular function. For instance, changes in intracellular amino acid pools can indicate shifts in metabolic activity or cellular stress responses, providing clues for understanding disease mechanisms or optimizing bioprocesses (e.g., recombinant protein production).

The demand for reliable, efficient amino acid assay tools has grown with the expansion of these research areas. Traditional methods for amino acid analysis often require complex sample preparation, specialized equipment (e.g., high-performance liquid chromatography), or lengthy incubation times—limiting their accessibility and throughput. This assay kit addresses these challenges by offering a simplified, cost-effective solution tailored for routine research use, enabling researchers to obtain accurate amino acid measurements with minimal technical barriers.