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| Product Name | Hydroxyproline (HYP) Content Assay Kit, 100T/96S |
| Catalog No. | AACAK-YJL-0018 |
| Detection Method | Micro-volume method, Spectrophotometer/Microplate Reader |
| Storage | Store at 2-8°C, 6 months |
| Intended Use | For research use only. |
| Note | For your safety and health, please wear lab coat, disposable gloves and mask during operation. |
Hydroxyproline (HYP) is a unique modified amino acid that exists almost exclusively in animal proteins—with collagen being its most prevalent carrier. Collagen, as a key structural protein of the extracellular matrix, is widely distributed in connective tissues such as skin, tendons, cartilage, bones, and blood vessels. It plays an irreplaceable role in maintaining tissue rigidity, tensile strength, and overall structural integrity, making it crucial for various biological processes and research fields.
The content of HYP is closely linked to collagen metabolism and fibrosis levels, which makes it a vital indicator for scientific research. Specifically:
In studies related to connective tissue health, HYP content reflects the synthesis and catabolism of collagen. For example, in research on wound healing, changes in HYP levels can indicate the progress of collagen deposition and tissue repair.
In fibrosis research (including liver fibrosis, renal interstitial fibrosis, and pulmonary fibrosis), collagen is the main component of fibrotic tissue. Since HYP is specific to collagen, detecting HYP content can effectively evaluate the degree of fibrosis, providing key data support for studying the pathogenesis of fibrotic diseases and screening potential therapeutic drugs.
In the field of tissue engineering and regenerative medicine, collagen is often used as a scaffold material for cell culture and tissue transplantation. Measuring HYP content helps researchers assess the quality and stability of collagen-based materials, ensuring their suitability for experimental or application purposes.
In studies on aging and dermatology, collagen loss is a major factor contributing to skin aging (such as wrinkles and reduced elasticity). HYP detection can quantify collagen levels in skin tissue, aiding in the research of anti-aging mechanisms and the development of related skincare research models.
Adopts a micro-volume detection method compatible with both spectrophotometers and microplate readers, offering flexibility in instrument selection to adapt to different laboratory setups.
Supports 100 tests (or 96-well plate format) in one kit, which is suitable for batch sample detection—saving time and reducing the operational error caused by repeated reagent preparation.
Requires simple storage conditions (2-8°C) and has a 6-month shelf life, making it easy for laboratories to manage inventory without frequent restocking.
The assay process is designed with user safety in mind; when operated following standard procedures (wearing lab coats, disposable gloves, and masks), it minimizes potential risks during sample handling and reagent use.
Is compatible with common biological sample types used in collagen/HYP research (such as animal tissues, cells, serum, and plasma), eliminating the need for specialized sample preprocessing tools and expanding its application scope in different research scenarios.
High reliability: The kit’s detection principle is based on well-validated biochemical reactions for HYP quantification, ensuring consistent and accurate results across repeated experiments—helping researchers obtain credible data for their studies.
Operational convenience: The micro-volume method reduces the amount of sample and reagent required per test, lowering experimental costs while simplifying the operation process. Even researchers with basic laboratory skills can complete the assay by following the standard protocol.
Cost-effectiveness: With 100T/96S capacity per kit, the unit test cost is optimized, making it an economical choice for laboratories that conduct regular HYP detection, whether for small-scale preliminary experiments or large-scale sample screening.
Stable performance: The reagents in the kit are formulated to maintain stability under the recommended storage conditions (2-8°C) for 6 months. This stability ensures that the kit’s detection accuracy remains consistent throughout its shelf life, avoiding experimental deviations caused by reagent degradation.
For research use only, not for clinical use.
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