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| Product Name | Arginine (Arg) Content Assay Kit, 100T/96S |
| Catalog No. | AACAK-YJL-0017 |
| Detection Method | Micro-volume method |
| Storage | Store at 2-8°C, 6 months |
| Intended Use | For research use only. |
| Note | For your safety and health, please wear lab coat, disposable gloves and mask during operation. |
Arginine (Arg), a semi-essential amino acid, plays indispensable physiological roles in both human and animal organisms, making the accurate detection of its content critical for various research fields. Its multifaceted functions span key biological processes, underpinning the significance of reliable Arg quantification tools like this assay kit.
Core Role in Protein Metabolism: As a building block of proteins, Arginine is involved in protein synthesis and degradation, directly influencing cellular growth, repair, and function. For researchers studying tissue development, muscle physiology, or cellular response to stress, measuring Arg levels helps elucidate how this amino acid regulates protein homeostasis—an essential factor in understanding conditions like muscle wasting, wound healing, or age-related cellular decline.
Key Mediator of Nitric Oxide (NO) Synthesis: Within the body, Arginine is the primary precursor for NO production via the action of nitric oxide synthases (NOS). NO acts as a vital signaling molecule that relaxes vascular smooth muscle, regulates blood vessel elasticity, and supports endothelial function. This makes Arg content analysis critical for cardiovascular research, including studies on hypertension, atherosclerosis, and endothelial dysfunction. By quantifying Arg, researchers can assess substrate availability for NO synthesis and its impact on vascular health.
Regulation of Metabolic Pathways: Arginine contributes to glucose and lipid metabolism by stimulating the secretion of adrenal hormones. This hormonal modulation helps lower blood sugar levels—potentially restoring normal glucose ranges in hyperglycemic models—and reduces fatty acid production, offering insights for diabetes research, obesity studies, and metabolic syndrome investigations. Additionally, Arginine is involved in polyamine synthesis, which is essential for cell proliferation and differentiation, making it relevant for cancer research (e.g., studying tumor cell growth) and developmental biology.
Broad Research Applicability: The need to measure Arg content extends across multiple disciplines. In animal science, it supports studies on livestock nutrition and growth performance; in plant biology, it aids research on plant stress responses and nutrient metabolism; in clinical research, it provides data for exploring links between Arg levels and diseases like sepsis, kidney dysfunction, or immune disorders. This assay kit addresses these diverse research needs by enabling precise Arg quantification.
Versatile Detection Method: Utilizes the micro-volume method, which is compatible with common laboratory instruments (e.g., microplate readers, spectrophotometers), allowing seamless integration into existing research workflows without the need for specialized equipment.
Reliable Storage and Shelf Life: Maintains stability when stored at 2–8°C, with a 6-month shelf life. This eliminates the need for complex long-term storage conditions (e.g., -20°C freezers) and ensures consistent performance over an extended period, reducing reagent waste.
Broad Sample Compatibility: Validated for use with a wide range of sample types, including animal/plant tissues, bacterial cultures, cells, serum, plasma, and other biological fluids. This flexibility eliminates the need for multiple kits for different sample sources, simplifying experimental design.
User-Friendly Operation: Requires minimal specialized technical expertise, with clear protocols that streamline sample preparation and detection steps. Features like reduced incubation times and simple reagent mixing steps help save time and minimize human error during experiments.
High-Quality Reagent Formulation: The kit’s reagents are pre-optimized to ensure consistent reaction conditions, reducing variability between experiments. This reliability is critical for generating reproducible data—an essential requirement for peer-reviewed research and experimental validation.
Precise Quantification: Delivers accurate Arg content measurements by leveraging a well-characterized detection principle (based on specific chromogenic reactions in alkaline media, with absorbance read at 525 nm). This precision ensures that researchers can trust the data to draw valid conclusions about Arg levels in their samples.
Cost-Effective Packaging: Available in a 100T/96S format, which provides sufficient reagents for up to 100 tests (or 96 well-based assays). This size balances affordability with practicality, making it suitable for both small-scale experiments and medium-throughput research projects.
Safety-Focused Design: Includes clear safety guidelines (e.g., recommending lab coats, disposable gloves, and masks during operation) to protect users from potential reagent exposure. This attention to safety aligns with standard laboratory best practices and minimizes health risks.
Research-Only Optimization: Tailored specifically for research applications, with reagent concentrations and protocols optimized to meet the rigorous demands of scientific studies (e.g., high sensitivity for low Arg concentrations in biological samples). Unlike general-purpose kits, it avoids compromises that could limit research utility.
Time-Efficient Workflow: Reduces total experimental time compared to traditional Arg detection methods. The simplified sample processing (e.g., compatible with common homogenization tools) and fast reaction kinetics mean researchers can obtain results in a shorter timeframe, accelerating project progress.
For research use only, not for clinical use.
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