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| Product Name | Pro-Grade 2x NGS Library Amplification Premix (bulk pack available) |
| Catalog No. | REKI-0091 |
| Description | A pro-grade 2x amplification premix for sequencing library construction, combining high-fidelity polymerase, dNTPs, a library-optimised buffer and a protective agent that preserves activity through repeated freeze-thaw. Library validity is demonstrated end to end: the mix has been used with matching fragmentation and ligation modules and the resulting libraries sequenced on short-read platforms with clean insert profiles and balanced coverage. Bulk packaging to 2000 reactions supports production-scale library preparation. |
| Intended Use | Amplification of DNA sequencing libraries; whole-genome library preparation with sequencing-level validation. |
| Principle / Technology | High-fidelity polymerase with proofreading amplifies adapter-ligated libraries in a stabilised, library-optimised buffer. |
| Sample Type | Adapter-ligated DNA libraries |
| Performance Range / Specifications | High sensitivity and specificity; stable activity after repeated freeze-thaw; sequencing-validated library quality. |
| Specificity | Pro formulation with bulk packaging to 2000 reactions. |
| Reaction Conditions / Protocol | Add primers and library template to the premix and amplify. |
| Components / Formulation | High-fidelity DNA polymerase, dNTPs, optimised buffer, protective agent. |
| Storage Conditions | -25 to -15 °C |
| Shelf Life | 18 months |
| Package Specifications | 24 T, 96 T, 2000 T |
| Product Form | 2x premix solution |
| Key Features | End-to-end sequencing validation; freeze-thaw resilience; production-scale packs. |
| Concentration | 2x |
| Compatibility | Short-read sequencing library workflows |
| Readouts / Downstream Assays | Enriched sequencing libraries |
For research use only, not for clinical use.
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