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| Product Name | Plasma/Serum Protein Depletion Spin Column Kit, Top-14 Abundant Proteins |
| Catalog No. | ATR-SPS-0022 |
| Description | Immunoaffinity spin column kit depleting the 14 most abundant plasma proteins (>95% total protein mass) for enhanced detection of low-abundance biomarkers. Removes albumin, IgG, transferrin, fibrinogen, IgA, α2-macroglobulin, IgM, haptoglobin, apolipoproteins A-I/A-II, α1-antitrypsin, α1-acid glycoprotein, complement C3, and HDL. Spin column format — no LC system required. |
| Intended Use | Plasma/serum high-abundance protein depletion prior to proteomics; biomarker discovery; 2D-PAGE and LC-MS/MS dynamic range improvement; deep plasma proteome coverage. |
| Principle / Technology | Polyclonal antibodies to 14 abundant proteins immobilized on agarose resin; flow-through contains low-abundance proteome; >95% removal of targeted proteins |
| Detection Method | Dilute plasma 1:5; apply to pre-equilibrated column; incubate 10 min with rotation; centrifuge 1,000×g 2 min; collect flow-through; concentrate if needed |
| Sample Type | Human plasma (EDTA, heparin, citrate), human serum; 10-50 µL per column; pre-clear by centrifugation |
| Sensitivity / LOD | Depletion >95% targeted proteins; ~95% total protein removal; >90% recovery low-abundance proteins; capacity ~3 mg/column |
| Specificity | Specific for 14 high-abundance human plasma proteins; cross-reactivity with NHP ~80-90% |
| Reaction Conditions / Protocol | Equilibration: 5 min; depletion: 15 min; total <30 min for 12 samples |
| Components / Formulation | Immunoaffinity spin columns, equilibration/binding buffer (10×), wash buffer (10×), elution buffer (10×), collection tubes, 3 kDa concentrators (optional) |
| Storage Conditions | 2-8 °C; do not freeze columns; 0.05% sodium azide preservative |
| Shelf Life | 12 months at 2-8 °C |
| Package Specifications | 6, 12, 24 columns; each 10-50 µL plasma/serum |
| Product Form | Pre-packed spin columns with liquid buffers |
| Key Features | Removes 14 most abundant proteins; spin format — no LC needed; single-use; >90% low-abundance protein recovery; regenerable up to 20 cycles |
| Purity | Affinity-purified goat antibodies; capacity 3±0.5 mg/column; no detectable antibody leaching |
| Concentration | As specified on product label |
| Activity / Unit Definition | Sample processing efficiency ≥90% recovery per validation protocol |
| Molecular Weight | Not applicable or as specified for enzyme components |
| Source / Origin | Synthetic and recombinant components as specified |
| pH Range / Optimal pH | As stated in product specification |
| Shipping Conditions | Cold pack 2-8 °C; do not freeze; ship upright |
| Expiration Date / Stability | 12 months at 2-8 °C; do not use if bed dried |
| Regulatory / Compliance | For laboratory and research use only; RUO; contains sodium azide; ISO 9001 |
| Compatibility | Compatible with downstream PCR, mass spectrometry, and immunoassay workflows |
| Recommended Buffer System | Use as directed; components optimized for stated application |
| Application Notes / Precautions | Work quickly to prevent sample degradation; use appropriate protease/phosphatase inhibitors if needed |
| Batch-to-Batch Consistency | Protein precipitation efficiency and analyte recovery within ±15% of reference lot |
For research use only, not for clinical use.
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