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| Product Name | PIPES Buffer, 10 mM, pH 6.8 |
| Catalog No. | RERE-0429 |
| Description | Cell cycle analysis by flow cytometry needs a medium that fixes, permeabilises and buffers in one step, and this PIPES formulation does exactly that. The 10 mM PIPES anchors pH 6.8, sodium chloride and magnesium chloride manage ionic strength, and Triton X-100 gently perforates the membrane so DNA-binding dyes can reach the nucleus. The result is a preparation step that leaves DNA content measurement clean and reproducible. |
| Intended Use | Cell preparation for flow cytometric cell cycle and DNA content analysis. |
| Principle / Technology | PIPES buffering with mild detergent permeabilisation exposes nuclear DNA to staining dyes. |
| Specificity | 10 mmol/L PIPES; with sodium chloride, magnesium chloride and Triton X-100; pH 6.8. |
| Reaction Conditions / Protocol | Use as the fixation and permeabilisation buffer in cell cycle staining protocols. |
| Storage Conditions | 2-8°C; 6 months. |
| Package Specifications | 100 mL |
| Key Features | Combined buffer and permeabiliser; tuned to cell cycle workflows; single-bottle convenience. |
For research use only, not for clinical use.
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