| Product Name | Phosphate Assay Reagent |
| Catalog No. | CMTR-HMM-0016 |
| Description | Phosphate is one of the most important inorganic ions in biological systems. It plays multiple roles. One of its most important functions is as a molecular switch, activating or deactivating enzyme activity by mediating various protein kinases and phosphatases in biological systems. |
| Application | This product is used to measure phosphate levels. |
| Principle | A proprietary formulation of malachite green and ammonium molybdate forms a chromogenic complex with phosphate ions, producing a strong absorption band at approximately OD = 650 nm. |
| Assay Type | Quantitative |
| Detection Method | Colorimetric |
| Applicable Instruments | Microplate reader |
| Detection Time | 2 h |
| Sample Type | Cell culture media, Other biological fluids, Cell lysate, Serum, Plasma, Urine, Tissue extracts |
| Detection Limit | 1 µM |
| Standard Linear Range | 0.001 mM - 1 mM |
| Procedure | 1. Add the reaction mixture to the sample and standard wells. 2. Incubate for 30 minutes. 3. Analyze using a microplate reader. |
| Storage | 4°C |
For research use only, not for clinical use.
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