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| Product Name | Phenylmethylsulfonyl Fluoride (PMSF) |
| Catalog No. | RERE-0029 |
| Description | PMSF is an irreversible inhibitor of serine proteases such as chymotrypsin, trypsin and thrombin, acting by sulfonylation of the active-site serine residue. It also inhibits cysteine proteases such as papain (an effect reversed by DTT) and mammalian acetylcholinesterase. Supplied as a crystalline powder that is poorly soluble in water, it should be dissolved in methanol, ethanol or isopropanol to prepare a stock solution and added to lysis buffers immediately before use. |
| Intended Use | Protease inhibition during protein extraction from cells and tissues; often combined with other protease inhibitors or phosphatase inhibitors such as sodium orthovanadate |
| Principle / Technology | Irreversible sulfonylation of the active-site serine residue of serine proteases, abolishing enzymatic activity |
| Detection Method | Chymotrypsin, trypsin, thrombin and other serine proteases; papain-like cysteine proteases; acetylcholinesterase |
| Sample Type | Cell and tissue lysates prepared for protein analysis |
| Performance Range / Specifications | Inhibits proteolysis during extraction when added fresh to lysis buffers at 0.1 to 1 mM |
| Specificity | Serine protease specificity with additional activity against papain-like cysteine proteases and acetylcholinesterase |
| Reaction Conditions / Protocol | Prepare a 200 mM stock in methanol, ethanol or isopropanol; add to the lysis buffer immediately before use at 0.1 to 1 mM final concentration |
| Components / Formulation | Crystalline PMSF powder, 1 g per vial |
| Storage Conditions | Powder at 2 to 8°C for 2 years; stock solutions held at 4°C or aliquoted at -20°C remain stable for several months |
| Shelf Life | 24 months as supplied |
| Package Specifications | 1 g; 5 x 1 g |
| Product Form | White to greyish-white crystalline powder |
| Quality Control | Identity and purity verified by the manufacturing lot record; purity at least 98.5% |
| Key Features | Irreversible serine protease inhibitor; broad protection during extraction; freely soluble in common organic solvents; economical powder format |
| Purity | At least 98.5% |
| Activity / Unit Definition | Irreversible inhibition of serine protease active sites |
| Molecular Weight | 174.2 |
| Source / Origin | Chemical synthesis |
| Shipping Conditions | Ambient temperature |
| Expiration Date / Stability | Stable as dry powder for 24 months at 2 to 8°C; unstable in aqueous solution and must be added fresh |
| Regulatory / Compliance | For research use only |
| Compatibility | Compatible with protein extraction workflows feeding into SDS-PAGE, Western blotting and immunoprecipitation |
| Application Notes / Precautions | Toxic and corrosive; wear gloves and eye protection; prepare aqueous working solutions immediately before use |
| Batch-to-Batch Consistency | Lot-numbered packaging; lot record retained for traceability |
| Delivery Standard | Standard catalogue shipment, packaging integrity verified before dispatch |
| Readouts / Downstream Assays | Protein extraction followed by Western blotting, immunoprecipitation or general protein analysis |
For research use only, not for clinical use.
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