| Product Name | Pepsin Activity Assay Reagent (Microplate Reader) |
| Catalog No. | ETR-HMM-0010 |
| Description | Gastric pepsin is secreted by the chief cells of the gastric mucosa and can break down proteins in food into peptides and amino acids such as tyrosine and phenylalanine. Its activity measurement can be used to distinguish between neurogenic hypochlorhydria and gastric hypochlorhydria. A decrease in gastric pepsin secretion may also occur in conditions such as chronic gastritis, chronic gastric dilatation, and chronic duodenitis. |
| Application | Pepsin can catalyze the hydrolysis of hemoglobin to produce tyrosine, with the product exhibiting a characteristic absorption peak at 275 nm. The activity of pepsin can be characterized by changes in absorbance. |
| Applicable Instruments | Microplate Reader |
| Number of Testable Samples | 50 Samples |
| Matching | 96-well plate |
| Detection Time | 4 h (50 Samples) |
| Detection Method | Tyrosine colorimetric method |
| Spectral Parameters | 275 nm |
| Signal Response | Incremental |
| Notes | If the A measurement is greater than 1.2, it is recommended to dilute the crude enzyme solution appropriately before proceeding with the measurement, and make corresponding adjustments during calculation. |
For research use only, not for clinical use.
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