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| Product Name | PCR Instrument Heat Block Cleaner, Nucleic Acid Contamination Removal |
| Catalog No. | ATRCS-0025 |
| Description | Specialized cleaning solution for thermal cycler heat blocks designed to eliminate amplifiable nucleic acid contamination that can cause false-positive signals in PCR workflows. The dual-action formula combines a mild alkaline detergent with a proprietary DNA/RNA degradation agent that hydrolyzes residual nucleic acid fragments without damaging the anodized aluminum block surface or Peltier elements. Suitable for routine maintenance between PCR runs and decontamination after high-copy-number template amplification. |
| Intended Use | Routine cleaning and nucleic acid decontamination of thermal cycler heat blocks, plate holders, and lid assemblies in PCR laboratories to prevent carryover contamination between amplification runs. |
| Principle / Technology | Alkaline hydrolysis of residual nucleic acid polymers combined with surfactant emulsification of protein and lipid residues; UV-fluorescence verification confirms cleaning efficacy. |
| Detection Method | Apply to heat block wells using lint-free swab; incubate 5 minutes; wipe clean with damp cloth; verify cleanliness with UV light or PCR blank run. |
| Sample Type | Thermal cycler heat blocks, heated lid assemblies, 96-well and 384-well block formats. |
| Performance Range / Specifications | Eliminates ≥99.9% of amplifiable DNA template as verified by qPCR using 1×10^6 copy challenge. |
| Sensitivity / LOD | Detectable reduction to less than 10 copies residual DNA per well after treatment as measured by qPCR. |
| Specificity | Targets nucleic acid polymers, protein films, and salt deposits; does not react with anodized aluminum or PEEK surfaces. |
| Reaction Conditions / Protocol | Apply 50-100 µL per well; incubate at room temperature for 5 minutes; wipe and air dry. |
| Components / Formulation | Alkaline buffer system, nuclease enzyme blend, nonionic surfactant, chelating agent, deionized water. |
| Storage Conditions | Store at 2-8 °C; protect from light; do not freeze. |
| Shelf Life | 12 months from date of manufacture when stored as directed. |
| Package Specifications | 50 mL, 250 mL, 500 mL dropper bottles or spray bottles. |
| Product Form | Clear, colorless liquid solution. |
| Quality Control | Each lot tested for nucleic acid removal efficacy using qPCR challenge with 1×10^6 copies of 200 bp amplicon; confirmed negative amplification after treatment. |
| Key Features | Dual-action cleaning and nucleic acid degradation; non-corrosive to thermal cycler block materials; UV-fluorescence indicator confirms treatment; compatible with all major thermal cycler brands. |
| Purity | Nuclease-free certified; bacterial endotoxin <0.05 EU/mL; DNase/RNase not detected. |
| Concentration | Ready-to-use formulation; no dilution required. |
| Activity / Unit Definition | Nucleic acid degradation capacity: ≥1 µg DNA per mL of solution within 5 minutes at 25 °C. |
| Molecular Weight | Not applicable — multi-component enzyme and buffer formulation. |
| Source / Origin | Recombinant nuclease enzymes expressed in E. coli; analytical grade buffer salts. |
| pH Range / Optimal pH | Optimal pH 9.0-9.5 for nuclease activity; working solution pH 9.2 ± 0.2. |
| Shipping Conditions | Cold pack recommended for summer transit; ambient acceptable for short transit (<72 hours). |
| Expiration Date / Stability | 12 months at 2-8 °C; after opening, use within 6 months. Discard if solution becomes turbid. |
| Regulatory / Compliance | For research use and laboratory maintenance; not for diagnostic procedures. |
| Compatibility | Compatible with anodized aluminum, gold-plated, and silver block surfaces; PEEK and PTFE components. Not recommended for uncoated copper heat blocks. |
| Recommended Buffer System | Tris-glycine alkaline buffer with EDTA chelating agent; pH stabilized for optimal nuclease function. |
| Application Notes / Precautions | Always run a no-template control (NTC) PCR after cleaning to verify decontamination. For heavy contamination, repeat treatment twice. Do not autoclave the cleaning solution as heat inactivates the nuclease enzymes. Use within 30 days of first opening. |
| Batch-to-Batch Consistency | Nuclease activity within ±15% of reference lot; pH within ±0.2 units; negative qPCR result after standard challenge. |
For research use only, not for clinical use.
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