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| Product Name | One-Tube Pathogen Amplicon Library Preparation Kit |
| Catalog No. | NG-QCY-0022 |
| Description | A closed-tube multiplex amplification and library preparation system designed to minimize handling, reduce contamination risk and generate sequencing-ready pathogen amplicon libraries. |
| Intended Use | Targeted next-generation sequencing of DNA and RNA pathogens. |
| Principle / Technology | Amplification and library construction are completed in one tube with a single PCR stage, avoiding intermediate opening and purification. |
| Detection Method | Targeted next-generation sequencing. |
| Sample Type | DNA samples and reverse-transcribed RNA pathogen samples. |
| Performance Range / Specifications | Supports 1–1,000-plex panels and 30–80% panel GC content; workflow as short as 3 hours with less than 1 hour of hands-on time; 24 or 96 reactions. |
| Sensitivity / LOD | Reported input sensitivity down to approximately 5 target copies. |
| Specificity | Closed-tube workflow and optimized multiplex chemistry support specific, uniform target amplification. |
| Reaction Conditions / Protocol | Add template and assay-specific primer pool to the one-tube reaction, complete the programmed amplification/library process, and proceed to sequencing-compatible cleanup and quality assessment as required. |
| Components / Formulation | One-tube multiplex amplification and library preparation reagents for targeted pathogen panels. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 24 reactions; 96 reactions. |
| Product Form | Multi-component targeted library preparation kit. |
| Key Features | One-tube workflow; one PCR stage; no intermediate purification; low aerosol contamination risk; low-copy detection; rapid processing. |
| Shipping Conditions | Ship at or below 0°C. |
| Compatibility | Compatible with Illumina and MGI sequencing platforms. |
| Application Notes / Precautions | Use panel-specific primers and validate index design, cleanup and sequencing depth for the intended assay. |
For research use only, not for clinical use.
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