One-Step Globin mRNA Removal Reagent for Human Blood RNA (4 min Reaction)
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One-Step Globin mRNA Removal Reagent for Human Blood RNA (4 min Reaction)

Cat.No: REKI-0057 Datasheet

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Product Name One-Step Globin mRNA Removal Reagent for Human Blood RNA (4 min Reaction)
Catalog No. REKI-0057
Description A targeted reagent for removing globin mRNA from human blood-derived RNA. Globin transcripts occupy roughly 30 to 70 percent of blood mRNA, draining sequencing depth from blood transcriptome experiments, so suppressing them during first-strand cDNA synthesis is an efficient way to raise informative data. The reagent blocks globin reverse transcription with high specificity while leaving other mRNA, non-coding RNA and circular RNA untouched.
Intended Use Globin mRNA removal ahead of mRNA and non-coding RNA library construction from blood samples.
Principle / Technology Targeting probes anneal to globin mRNA during first-strand cDNA synthesis and block its reverse transcription, selectively excluding it from the library.
Sample Type Human blood-derived total RNA, 100 pg to 1 ug
Performance Range / Specifications Reaction time about 4 min; single-step operation
Specificity Highly selective for globin mRNA with intact preservation of non-target RNA species
Storage Conditions Store at -25 to -15°C
Package Specifications 8 T / 24 T / 96 T
Product Form Probe-based reagent mix
Key Features Can be combined with a matching one-step rRNA removal reagent for joint rRNA and globin depletion; works with first-strand cDNA reagents from any supplier
Compatibility Pairs with first-strand cDNA synthesis reagents from any supplier

For research use only, not for clinical use.

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