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| Product Name | One-Step 3-Minute Human rRNA Removal Reagent (Locked Nucleic Acid Probe Method) |
| Catalog No. | REKI-0056 |
| Description | A targeted rRNA removal reagent that suppresses reverse transcription of human ribosomal RNA with locked nucleic acid modified probes during first-strand cDNA synthesis, without affecting cDNA generation from messenger, non-coding or circular RNA. Compared with conventional RNase H digestion it takes only 3 minutes, runs as a single step and preserves non-target RNA completely, simplifying workflows dramatically. |
| Intended Use | rRNA suppression before mRNA and non-coding RNA sequencing, RNA virus detection with host rRNA background reduction, and DNA/RNA co-library workflows. |
| Principle / Technology | Locked nucleic acid probes anneal to rRNA during reverse transcription and block its cDNA synthesis, selectively excluding ribosomal sequences from the library. |
| Sample Type | Total RNA and FFPE RNA, 100 to 1000 ng input |
| Performance Range / Specifications | Reaction time 3 min; single-step operation |
| Specificity | Highly selective suppression of rRNA with intact preservation of mRNA, non-coding RNA and circular RNA |
| Storage Conditions | Store at -25 to -15°C |
| Package Specifications | 8 T / 24 T / 96 T |
| Product Form | Probe-based reagent mix |
| Key Features | 3 minute one-step workflow; compatible with first-strand cDNA reagents from any supplier; strong performance on degraded FFPE RNA |
| Compatibility | Pairs with first-strand cDNA synthesis reagents from any supplier |
For research use only, not for clinical use.
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