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| Product Name | One-Pot Enzymatic DNA Fragmentation and End Repair Module (dual-platform) |
| Catalog No. | REKI-0088 |
| Description | A next-generation enzymatic library preparation reagent that merges fragmentation with end repair and A-tailing in a single pot, dispensing with ultrasonication entirely. Handling 500 pg to 1 ug of routine animal, plant or microbial genomic DNA, the high-quality fragmentation enzyme delivers stable, low-bias shearing while the accompanying activities prepare ligation-ready ends. Reaction products pass directly to adapter ligation, simplifying workflows and reducing cost and hands-on time for dual-platform genome sequencing. |
| Intended Use | Enzymatic fragmentation and end repair for animal, plant and microbial genome sequencing library construction. |
| Principle / Technology | Single-reaction enzymatic shearing coupled to end polishing and dA-tailing yields adapter-ready fragments. |
| Sample Type | Animal, plant and microbial genomic DNA |
| Performance Range / Specifications | Input 500 pg to 1 ug; stable low-bias fragmentation; single-pot convenience. |
| Specificity | Integrated fragmentation and end repair chemistry. |
| Reaction Conditions / Protocol | Combine genomic DNA with the premix, incubate to the target size, then ligate adapters without purification. |
| Components / Formulation | Fragmentation with end repair and A-tailing enzyme premix. |
| Storage Conditions | -25 to -15 °C |
| Shelf Life | 18 months |
| Package Specifications | 24 T, 96 T |
| Product Form | Enzyme and buffer premix |
| Key Features | Sonication-free workflow; low fragment bias; direct ligation handoff. |
| Compatibility | Dual short-read sequencing platforms |
| Starting Material / Tissue Site | 500 pg to 1 ug genomic DNA |
| Readouts / Downstream Assays | Adapter-ready fragmented DNA |
For research use only, not for clinical use.
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