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| Product Name | Nuclei Isolation Lysis Buffer for Animal Tissue (ATAC and CUT&Tag sample prep) |
| Catalog No. | RERE-0073 |
| Description | A dedicated lysis buffer that disrupts animal tissue or cultured cells while leaving nuclei intact, producing clean nuclear preparations for chromatin accessibility profiling and protein-DNA interaction assays. Gentle detergent conditions preserve nuclear membrane integrity so transposase and antibody access remains uniform across the preparation, and the buffer slots directly into standard ATAC or CUT&Tag protocols without additional crosslinking steps. |
| Intended Use | Preparation of intact nuclei from animal tissue or cells for ATAC chromatin accessibility assays and CUT&Tag protein-DNA interaction profiling. |
| Principle / Technology | Mild detergent lysis releases nuclei while preserving envelope integrity for subsequent enzymatic tagging reactions. |
| Sample Type | Animal tissue, cultured cells |
| Performance Range / Specifications | Good nuclear integrity after isolation as judged by downstream assay performance. |
| Specificity | Formulated for chromatin assays rather than whole-cell lysis. |
| Reaction Conditions / Protocol | Homogenise tissue or cells in the buffer, wash nuclei and proceed to transposition or antibody incubation. |
| Components / Formulation | Nuclei isolation lysis buffer. |
| Storage Conditions | -25 to -15 °C |
| Package Specifications | 10 mL, 200 mL |
| Product Form | Liquid buffer |
| Key Features | Intact-nuclei output; direct entry into ATAC and CUT&Tag protocols; scalable volumes. |
For research use only, not for clinical use.
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