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| Product Name | Nuclei Isolation Buffer, NP-40 Based, for ATAC-seq and ChIP-seq |
| Catalog No. | ATR-SPS-0013 |
| Description | A cold non-ionic detergent-based lysis buffer for the rapid isolation of intact nuclei from fresh and frozen tissues and cultured cells for epigenomic applications including ATAC-seq, ChIP-seq, and single-nucleus RNA-seq. |
| Intended Use | Isolating pure nuclei from cells and tissues while preserving chromatin structure and nuclear protein-DNA interactions for downstream epigenomic and transcriptomic analyses. |
| Principle / Technology | NP-40 detergent selectively solubilizes cytoplasmic and plasma membranes while leaving nuclear envelope intact; sucrose cushion or differential centrifugation separates nuclei from cytoplasmic debris. |
| Detection Method | Nuclear purity by DAPI staining and microscopy, chromatin integrity by Tn5 tagmentation efficiency |
| Sample Type | Fresh and frozen tissues, cultured cells, sorted cell populations |
| Performance Range / Specifications | Nuclear purity >95%; chromatin accessibility profile matching published ENCODE standards |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Homogenize tissue or resuspend cell pellet in ice-cold nuclei isolation buffer; Dounce homogenize 10–15 strokes; filter through 40 µm strainer; centrifuge at 500 × g for 5 min at 4°C; wash once. |
| Components / Formulation | Tris-HCl pH 7.4 10 mM, NaCl 10 mM, MgCl2 3 mM, NP-40 0.1%, protease inhibitor cocktail, in nuclease-free water |
| Storage Conditions | 2–8°C; add protease inhibitors fresh before use |
| Shelf Life | 12 months (without inhibitors) |
| Package Specifications | 50 mL, 100 mL |
| Product Form | Liquid, ready to use (inhibitors added fresh) |
| Quality Control | DNase/RNase free, nuclear yield and purity validation with mouse liver standard, chromatin quality assessment |
| Key Features | Optimized for ATAC-seq and ChIP-seq; preserves chromatin structure; rapid protocol; nuclease free; validated for 10x single-nucleus protocols |
For research use only, not for clinical use.
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