- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | NP-40 Nonionic Detergent for Protein Extraction |
| Catalog No. | RERE-0028 |
| Description | A nonionic polyethylene glycol-type surfactant of ethylphenol polymerised with ethylene oxide units. Its mild lysis strength permeabilises animal cells and tissues gently, releasing cytoplasmic and membrane-associated proteins while leaving the harshest nuclear extraction to stronger formulations, which makes it a go-to reagent when preserving protein complexes and activity matters more than exhaustive recovery. |
| Intended Use | Mild lysis of animal cells and tissues ahead of Western blotting, immunoprecipitation and general protein extraction. |
| Principle / Technology | Nonionic detergent action: the polyethylene glycol chain solubilises membranes with minimal disruption of protein-protein interactions. |
| Sample Type | Cultured animal cells and animal tissues |
| Performance Range / Specifications | Mild lysis strength preserving complexes and activity; typical working concentrations set by the downstream protocol |
| Specificity | Nonionic character with low denaturing tendency |
| Reaction Conditions / Protocol | Dilute into the lysis buffer at the protocol concentration, add to washed cells or tissue, incubate with mixing and clarify the lysate by centrifugation before downstream use. |
| Components / Formulation | Liquid detergent reagent |
| Storage Conditions | Room temperature in a dry place |
| Package Specifications | 100 mL and 500 mL bottles |
| Product Form | Viscous clear liquid |
| Quality Control | Released against identity and formulation criteria |
| Key Features | Gentle nonionic lysis that keeps complexes intact; broadly compatible with Western and IP buffer systems; supplied in convenient bottle sizes for routine extraction work |
| Purity | Biochemical grade |
| Concentration | Supplied as concentrated liquid for dilution into working buffers |
| Molecular Weight | Polymeric |
| Source / Origin | Synthetic nonionic surfactant |
| pH Range / Optimal pH | Neutral in solution |
| Shipping Conditions | Room temperature |
| Expiration Date / Stability | Stable at room temperature when kept dry |
| Regulatory / Compliance | Research-use reagent |
| Compatibility | Compatible with common lysis buffers and detergent exchange workflows; pairs with stronger detergents when nuclear fractions are needed |
| Application Notes / Precautions | Mix gently to avoid foaming; confirm lot performance when switching between detergent grades; store tightly capped and dry. |
| Batch-to-Batch Consistency | Lot-numbered bottles |
| Model Type | Nonionic surfactant reagent |
| Starting Material / Tissue Site | Cultured animal cells or tissues for mild extraction |
| Delivery Standard | Shipped at ambient temperature |
| Readouts / Downstream Assays | Downstream Western blot or immunoprecipitation of the prepared lysate |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
