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| Product Name | Nonionic Surfactant X-114, Molecular Biology Grade |
| Catalog No. | RERE-0033 |
| Description | This nonionic surfactant has a cloud point of 23°C: below that temperature it mixes freely with water, while above it the solution separates into aqueous and detergent phases. The transition underpins its classic use in biochemical workflows for separating hydrophilic proteins from lipoproteins, most prominently the extraction of membrane proteins. The molecular-biology-grade liquid is supplied neat with a critical micelle concentration near 0.2 mM. |
| Intended Use | Separation of aqueous-phase proteins from lipoproteins; extraction of membrane proteins by cloud-point phase separation |
| Principle / Technology | Temperature-driven phase partitioning above the 23°C cloud point segregates hydrophobic membrane proteins into the detergent phase |
| Sample Type | Protein extracts containing membrane or lipid-associated fractions |
| Performance Range / Specifications | Sharp phase transition at 23°C with reproducible partitioning between lots |
| Specificity | Enriches hydrophobic and membrane-associated proteins in the detergent phase |
| Reaction Conditions / Protocol | Mix the extract with surfactant below the cloud point, then warm above 23°C and centrifuge to separate the phases |
| Components / Formulation | Surfactant liquid, 500 mL per bottle |
| Storage Conditions | Room temperature |
| Package Specifications | 500 mL |
| Product Form | Clear to slightly hazy liquid at room temperature |
| Quality Control | Density verified at 1.058 g per mL at 25°C; cloud point confirmed at 23°C |
| Key Features | Molecular biology grade; defined cloud point of 23°C; critical micelle concentration near 0.2 mM; convenient large-volume format |
| Purity | Molecular biology grade |
| Concentration | Supplied neat; CMC approximately 0.2 mM (0.009% w/w in water) |
| Molecular Weight | 537 (average, n = 7 or 8) |
| Source / Origin | Chemical synthesis |
| Shipping Conditions | Ambient temperature |
| Expiration Date / Stability | Stable when stored at room temperature |
| Regulatory / Compliance | For research use only |
| Compatibility | Suited to membrane proteomics workflows and downstream electrophoresis of detergent-phase fractions |
| Application Notes / Precautions | Irritant; avoid skin and eye contact; warm gently and evenly to obtain a clean phase separation |
| Batch-to-Batch Consistency | Lot-numbered packaging; lot record retained for traceability |
| Delivery Standard | Standard catalogue shipment, packaging integrity verified before dispatch |
| Readouts / Downstream Assays | Membrane protein extraction, hydrophobic versus hydrophilic protein partitioning |
For research use only, not for clinical use.
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