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| Product Name | Nonionic Surfactant X-100, Molecular Biology Grade |
| Catalog No. | RERE-0032 |
| Description | This nonionic surfactant, structurally related to octylphenol ethoxylates, promotes the solubilisation of lipids and proteins across a wide range of molecular biology and immunology workflows. Typical uses include cell lysis, protein extraction, preparation of enzyme stock and protein-stabilising solutions, and permeabilisation steps in immunocytochemistry and immunohistochemistry. The molecular-biology-grade liquid is colourless to pale yellow and slightly hazy in appearance. |
| Intended Use | Cell lysis and protein extraction; membrane protein solubilisation; permeabilisation for ICC and IHC; formulation of enzyme storage solutions |
| Principle / Technology | Nonionic detergent micelles solubilise membrane lipids and hydrophobic protein regions without denaturing protein complexes |
| Sample Type | Cultured animal cells, tissues and membrane preparations |
| Performance Range / Specifications | Purity above 98% with consistent micelle-forming behaviour between lots |
| Specificity | Nonionic character preserves protein-protein interactions during extraction |
| Reaction Conditions / Protocol | Dilute into the desired lysis or washing buffer at the working concentration specified by the application |
| Components / Formulation | Surfactant liquid, 20 mL or 500 mL per bottle |
| Storage Conditions | Room temperature for storage and shipping |
| Package Specifications | 20 mL; 500 mL |
| Product Form | Colourless or pale yellow, slightly hazy liquid |
| Quality Control | Purity above 98%; density verified at 1.065 g per mL at 25°C |
| Key Features | Molecular biology grade; nonionic and protein-compatible; versatile across lysis, extraction and permeabilisation applications |
| Purity | Above 98% |
| Concentration | Supplied neat; dilute to the working concentration required |
| Molecular Weight | 647 |
| Source / Origin | Chemical synthesis |
| Shipping Conditions | Ambient temperature |
| Expiration Date / Stability | Stable when stored at room temperature |
| Regulatory / Compliance | For research use only |
| Compatibility | Compatible with immunoprecipitation, Western blotting, enzyme storage formulations and histological permeabilisation |
| Application Notes / Precautions | Irritant; avoid contact with skin and eyes; viscous liquid, pipette slowly for accurate volume transfer |
| Batch-to-Batch Consistency | Lot-numbered packaging; lot record retained for traceability |
| Delivery Standard | Standard catalogue shipment, packaging integrity verified before dispatch |
| Readouts / Downstream Assays | Protein extraction, immunocytochemistry, immunohistochemistry, enzyme formulation |
For research use only, not for clinical use.
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