| Product Name | Neutral Protease (NP) Activity Assay Reagent (Visible Spectrophotometer) |
| Catalog No. | ETR-HMM-0003 |
| Description | Neutral proteases (NP) are a class of proteases that act on protein peptide bonds in a neutral environment, hydrolyzing proteins into amino acids, peptides, and free amino acids. Due to their safety, non-toxicity, strong hydrolytic capacity, broad application range, and mild reaction conditions, they have widespread applications in the food industry, dairy processing, feed, cosmetics, and nutritional supplements. |
| Application | Neutral protease can catalyze the hydrolysis of casein to produce tyrosine under neutral conditions. Tyrosine can reduce phosphomolybdate compounds to form tungsten blue under alkaline conditions. The product has a characteristic absorption peak at 680 nm, and the activity of neutral protease can be characterized by changes in absorbance. |
| Applicable Instruments | Visible Spectrophotometer |
| Number of Testable Samples | 25 Samples |
| Matching | 1 mL glass cuvette (d=10 mm) |
| Detection Time | 4 h (25 Samples) |
| Detection Method | Folin method |
| Spectral Parameters | 680 nm |
| Signal Response | Incremental |
| Standard | Tyrosine |
| Reference Standards | y=1.6904x+0.0083 (R2=0.9999) |
| Standard Linear Range | 0.03125-0.75 μmol/mL |
| Notes | If the ∆A measurement difference is small, the reaction time in the first step can be appropriately extended (15-25 min), and the calculation should be modified accordingly. |
For research use only, not for clinical use.
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