| Product Name | Neutral Protease (NP) Activity Assay Reagent (Microplate Reader) |
| Catalog No. | ETR-HMM-0004 |
| Description | Neutral protease (NP) is a type of protease that acts on protein peptide bonds in a neutral environment, hydrolyzing proteins into amino acids, peptides, and free amino acids. Due to its safety, non-toxicity, strong hydrolysis capability, broad application range, and mild reaction conditions, it has widespread applications in the food industry, dairy processing, feed production, cosmetics, and nutritional supplements. |
| Application | Neutral protease can catalyze the hydrolysis of casein to produce tyrosine under neutral conditions. Tyrosine can reduce phosphomolybdate compounds to form tungsten blue under alkaline conditions. The product has a characteristic absorption peak at 680 nm, and the activity of neutral protease can be characterized by changes in absorbance. |
| Applicable Instruments | Microplate Reader |
| Number of Testable Samples | 50 Samples |
| Matching | 96-well plate |
| Detection Time | 5 h (50 Samples) |
| Detection Method | Folin method |
| Spectral Parameters | 680 nm |
| Signal Response | Incremental |
| Standard | Tyrosine |
| Reference Standards | y=0.8108x+0.0072 (R2=0.9999) |
| Standard Linear Range | 0.05-1.2 μmol/mL |
| Notes | If the ∆A measurement difference is small, the reaction time in the first step can be appropriately extended (15-25 min), and the calculation should be modified accordingly. |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2025 Alta DiagenoTech. All rights reserved.
