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| Product Name | Native Barcode Ligation Module for Nanopore Sequencing |
| Catalog No. | REKI-0117 |
| Description | A ligation module dedicated to the native barcode attachment step in nanopore library construction. The ligase raw material has been directionally engineered to accept end-prepared fragments carrying a 5-prime phosphate and 3-prime dA overhang and join native barcodes quickly, compressing the indexing step and keeping lot-to-lot library stability tight. Rigorous functional QC on every release protects reproducibility across projects. |
| Intended Use | Native barcode attachment in nanopore DNA library construction, sample multiplexing for long-read sequencing, rapid ligation workflows. |
| Principle / Technology | An engineered ligase joins native barcodes to 5-prime-phosphorylated, 3-prime-dA-overhang fragments with high efficiency at short incubation times. |
| Performance Range / Specifications | Rapid barcode joining onto end-prepared substrates; QC-verified lot stability. |
| Reaction Conditions / Protocol | Mix end-prepared DNA, native barcodes and ligation mix, incubate, then continue adapter attachment. |
| Components / Formulation | Engineered ligation enzyme, ligation buffer. |
| Storage Conditions | -25 to -15 °C |
| Package Specifications | 8 T, 24 T, 96 T |
| Product Form | Liquid reagents |
| Key Features | Directionally engineered ligase raw material; short incubation; drop-in compatible with upstream end-repair modules. |
| Compatibility | Nanopore (ONT) sequencing platform; pairs with companion end repair and adapter ligation modules |
For research use only, not for clinical use.
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