Nanopore DNA Library Preparation Kit V2

Name: Nanopore DNA Library Preparation Kit V2
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Cat.No: NG-QCY-0034 Datasheet

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Product Name	Nanopore DNA Library Preparation Kit V2
Catalog No.	NG-QCY-0034
Description	A ligation-based DNA library preparation kit for single-sample or barcoded nanopore sequencing of genomic DNA and amplicons.
Intended Use	Third-generation nanopore sequencing of animal, plant and microbial DNA samples.
Principle / Technology	High-activity repair, end-preparation and ligation enzymes convert DNA fragments into adapter-ligated libraries for nanopore sequencing.
Detection Method	Nanopore sequencing.
Sample Type	Genomic DNA and PCR amplicons from animals, plants and microorganisms.
Performance Range / Specifications	24 reactions; modular end-repair/A-tailing and optional DNA-damage repair workflow.
Specificity	Optimized end preparation and adapter ligation improve conversion of qualified DNA into sequencing-ready molecules.
Reaction Conditions / Protocol	Assess input DNA quality, perform end repair and A-tailing with optional damage repair, ligate nanopore adapters, clean up the library and load it onto the selected flow cell.
Components / Formulation	DNA repair, end-repair/A-tailing, adapter-ligation and library cleanup reagents.
Storage Conditions	Store at −30 to −15°C.
Package Specifications	24 reactions.
Product Form	Multi-module ligation library preparation kit.
Key Features	High-activity mutant enzymes; optimized repair and ligation buffers; modular damage-repair option; supports genomic DNA and amplicons; single or multiplex libraries.
Shipping Conditions	Ship at or below 0°C.
Compatibility	Compatible with MinION, GridION, PromethION and Flongle instruments and with SQK-LSK114-format nanopore workflows.
Application Notes / Precautions	High-molecular-weight input quality and gentle handling are important for long-read performance; validate barcode and flow-cell compatibility before use.

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For research use only, not for clinical use.