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| Product Name | Mycobacterium tuberculosis Rifampicin-Resistance PCR-Sanger Kit for 88 rpoB Mutations |
| Catalog No. | CS-DB-0034 |
| Description | The Alta DiagnoTech rifampicin-resistance mutation kit combines PCR with Sanger sequencing to analyze the 81 bp rpoB region spanning codons 507-533 in sputum specimens. The stated coverage includes up to 88 mutation types and several common substitutions. |
| Intended Use | Detection of Mycobacterium tuberculosis rpoB mutations associated with rifampicin resistance. |
| Principle / Technology | PCR amplification followed by Sanger sequencing, with UNG/UDG and dUTP carryover-contamination control. |
| Detection Method | PCR-Sanger sequencing |
| Sample Type | Sputum specimens |
| Performance Range / Specifications | Coverage of the 81 bp rpoB region spanning codons 507-533 and up to 88 mutation types; results can be produced in approximately 24 hours. |
| Components / Formulation | UNG/UDG enzyme and dUTP contamination-control system. |
| Key Features | rpoB codons 507-533 coverage Up to 88 mutation types Approximately 24-hour result time UNG/UDG-dUTP carryover prevention |
| Product Type | Tuberculosis drug-resistance mutation detection kit |
| Analytes / Targets | Mycobacterium tuberculosis rpoB rifampicin-resistance region, codons 507-533 |
| Genotypes / Variants Detected | Up to 88 mutation types, including 516 GAC→GTC, 526 CAC→TAC/CGC and 531 TCG→TTG |
For research use only, not for clinical use.
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