- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Multi-Target CUT&Tag Library Prep Kit with Nanobody-Tn5 (single sample, dual targets) |
| Catalog No. | REKI-0083 |
| Description | A library preparation kit for multi-target CUT&Tag on short-read sequencing platforms, covering 100 to 100,000 starting cells. The kit employs nanobody-fused Tn5 transposase that binds mouse and rabbit primary antibodies directly, so a single experiment can profile two targets from one cell sample and no secondary antibody is required. Higher signal-to-noise than conventional workflows, split Box I and Box II modules for room-temperature and frozen components, and a streamlined antibody incubation to transposition sequence make multi-epitope chromatin profiling practical on limited samples. |
| Intended Use | Simultaneous profiling of two protein-DNA interaction targets from one sample; histone modification and transcription factor studies; single-cell ChIP alternatives. |
| Principle / Technology | Nanobody-fused Tn5 transposase binds primary antibodies on chromatin and inserts sequencing adapters upon activation, converting bound DNA into indexed libraries in situ. |
| Sample Type | Cultured cells or dissociated animal cells (100-100,000 cells) |
| Performance Range / Specifications | 100 to 100,000 cell input; two targets per experiment; no secondary antibody; high signal-to-noise. |
| Specificity | Anti-mouse and anti-rabbit nanobody-Tn5 transposase modules. |
| Reaction Conditions / Protocol | Capture cells on lectin beads, incubate primary antibodies, bind nanobody-Tn5, activate transposition and amplify indexed libraries. |
| Components / Formulation | Concanavalin A beads, antibody incubation reagents, nanobody-Tn5 transposase, amplification reagents in two boxed modules. |
| Storage Conditions | Box I at 2 to 8 °C; Box II at -25 to -15 °C |
| Package Specifications | 4 T, 12 T, 48 T |
| Product Form | Boxed reagent modules |
| Key Features | Two targets per sample; secondary-antibody-free; sample-sparing workflow with high signal-to-noise. |
| Readouts / Downstream Assays | Multi-target chromatin interaction libraries |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
