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| Product Name | mRNA Capture and Purification Bead Kit V2 (Oligo(dT), inhibitor-tolerant) |
| Catalog No. | REKI-0093 |
| Description | An upgraded magnetic-bead kit for purifying polyadenylated RNA from 10 ng to 4 ug of good-quality total RNA across animal, plant and fungal material. Micron-scale paramagnetic beads coupled to Oligo(dT) capture mRNA through its poly(A) tail, stripping rRNA and inhibitors to deliver clean template for library construction. The chemistry holds its yield on difficult matrices where mRNA purification usually collapses, including traditional herbal material and polysaccharide, polyphenol or tumour-rich samples, with low rRNA carryover. An automation pack supports liquid-handling workflows. |
| Intended Use | mRNA purification before transcriptome library construction; RNA immunoprecipitation workflows; m6A modification studies; mRNA vaccine preparation. |
| Principle / Technology | Oligo(dT)-coupled paramagnetic beads hybridise to the poly(A) tail of mRNA, allowing magnetic separation from rRNA and contaminants. |
| Sample Type | Total RNA from animal, plant and fungal sources |
| Performance Range / Specifications | Input 10 ng to 4 ug total RNA; high purity and low rRNA residue on inhibitor-rich matrices. |
| Specificity | Poly(A)-tail targeted capture across eukaryotic RNA. |
| Reaction Conditions / Protocol | Hybridise beads to total RNA, wash on a magnet and elute purified mRNA. |
| Components / Formulation | Oligo(dT) mRNA capture beads with hybridisation and wash buffers. |
| Storage Conditions | 2 to 8 °C |
| Package Specifications | 8 T, 24 T, 96 T, 96 T (automation) |
| Product Form | Magnetic bead kit |
| Key Features | Inhibitor-tolerant chemistry; automation pack; broad eukaryote compatibility. |
| Starting Material / Tissue Site | 10 ng to 4 ug total RNA |
| Readouts / Downstream Assays | Purified mRNA for sequencing and modification studies |
For research use only, not for clinical use.
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