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| Product Name | Mouse Neutrophil Isolation Kit |
| Catalog No. | RESK-0472 |
| Description | The Mouse Neutrophil Isolation Kit (Negative Isolation) is designed for the isolation of neutrophils from single-cell suspensions of mouse bone marrow, spleen, or peripheral blood by negative selection. Non-target cells (non-CD11b+Ly-6G+ cells) are labeled with a biotin-conjugated monoclonal antibody cocktail and then depleted with streptavidin magnetic beads, yielding untouched, bead-free mouse neutrophils. Key features: Easy to use: no separation column required; isolation is achieved with a magnetic separator. High purity: isolated cell purity can reach over 95%. Excellent cell activity: negative isolation keeps magnetic beads from directly contacting target cells, so isolated cells are highly active and fully functional for downstream experiments. Fast: isolation can be completed in as little as 30 min. |
| Intended Use | For the isolation of neutrophils from single-cell suspensions of mouse bone marrow, spleen, or peripheral blood by negative selection, for research use. |
| Principle / Technology | Negative (untouched) magnetic isolation. A biotin-labeled monoclonal antibody cocktail binds non-target cells (non-CD11b+Ly-6G+ cells); streptavidin-labeled magnetic beads then capture and remove the labeled non-target cells on a magnetic separator, leaving bead-free mouse neutrophils in the supernatant. |
| Sample Type | Single-cell suspensions of mouse bone marrow, spleen, or peripheral blood |
| Performance Range / Specifications | Sorting capacity: 10 T: up to 1x10^8 cells 50 T: up to 5x10^8 cells 100 T: up to 1x10^9 cells Purity: over 95% Isolation time: as fast as 30 min |
| Specificity | Specifically isolates mouse CD11b+Ly-6G+ neutrophils; the negative antibody cocktail targets all other cell populations for depletion. |
| Reaction Conditions / Protocol | Negative isolation workflow (column-free): 1. Prepare a single-cell suspension from the appropriate sample. 2. Incubate the cells with the biotin-labeled negative antibody cocktail to label non-target cells. 3. Add streptavidin magnetic beads to capture the labeled non-target cells. 4. Place the tube on a magnetic separator; non-target cells are retained by the magnet. 5. Collect the supernatant containing the untouched, bead-free target cells. The entire isolation can be completed in as little as 30 min. |
| Components / Formulation | Mouse Neutrophil Negative Antibody Cocktail: 20 μL (10 T) / 100 μL (50 T) / 200 μL (100 T) Streptavidin Magnetic Beads: 0.2 mL (10 T) / 1 mL (50 T) / 2x 1 mL (100 T) |
| Storage Conditions | Store at 2-8 °C. Do not freeze. |
| Shelf Life | 1 year when stored at 2-8 °C. |
| Package Specifications | 10 T / 50 T / 100 T |
| Product Form | Liquid kit containing a biotin-labeled antibody cocktail and a streptavidin magnetic bead suspension |
| Quality Control | Purity verified by flow cytometry. In representative testing, CD11b+Ly6G+ neutrophils isolated from different mouse samples reached purities of 95.2%, 91.8% and 93.8%, respectively. |
| Purity | Isolated cell purity can reach over 95% |
| Expiration Date / Stability | Stable for 1 year when stored at 2-8 °C; do not freeze. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Designed for use with a magnetic separator; no separation column required. Best results are obtained with a 5 mL metal magnetic rack. A magnetic rack with a field strength greater than 7500 gauss is recommended. |
| Application Notes / Precautions | Do not freeze the kit components. |
For research use only, not for clinical use.
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