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| Product Name | Mouse Naive CD4+ T Cell Isolation Kit |
| Catalog No. | ATR-SPS-0038 |
| Description | A magnetic negative-selection kit for isolating naive CD4+ T cells from mouse lymphoid tissues without labeling the target population. Unwanted cells are tagged with biotinylated antibodies and removed using streptavidin magnetic beads. |
| Intended Use | Isolation of naive CD4+ T cells from mouse spleen and lymph-node single-cell suspensions. |
| Principle / Technology | Column-free negative selection using biotinylated monoclonal antibodies and streptavidin-coated magnetic beads. |
| Detection Method | Magnetic cell separation followed by flow-cytometric purity assessment. |
| Sample Type | Mouse spleen, lymph node and mixed spleen/lymph-node single-cell suspensions. |
| Performance Range / Specifications | Typical separation time: approximately 18 minutes; reported target-cell purity: 90-96%; reported viability: 90-96%; package capacity: up to 1 × 10^9 cells. |
| Reaction Conditions / Protocol | Label non-target cells with the antibody cocktail, add streptavidin magnetic beads and retain the unlabeled naive CD4+ T-cell fraction after magnetic separation. |
| Components / Formulation | Biotinylated cell-isolation antibody cocktail and streptavidin magnetic beads. |
| Storage Conditions | Store at 2-8°C. Do not freeze. |
| Package Specifications | For up to 1 × 10^9 cells. |
| Product Form | Column-free magnetic cell-isolation kit. |
| Quality Control | Purity and viability are assessed by flow cytometry. |
| Key Features | Approximately eighteen-minute workflow; no separation column; bead-free target cells; no abnormal activation; good yield and sample compatibility. |
| Shipping Conditions | Ship with ice packs. |
| Compatibility | Compatible magnetic cell-separation devices, flow cytometry, cell culture and downstream functional assays. |
| Application Notes / Precautions | Prepare a uniform single-cell suspension and remove clumps before magnetic separation. Maintain appropriate temperature and gentle handling throughout the procedure. |
For research use only, not for clinical use.
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