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| Product Name | Mouse CD8+ T Cell Isolation Kit |
| Catalog No. | RESK-0471 |
| Description | The Mouse CD8+ T Cell Isolation Kit (Negative Isolation) is designed for the isolation and purification of CD8+ T cells from mouse lymph nodes, spleen, or mixed samples thereof by negative selection. Non-target cells (non-CD8+ T cells) are labeled with a biotin-conjugated monoclonal antibody cocktail and then depleted with streptavidin magnetic beads, yielding untouched, bead-free mouse CD8+ T cells. Key features: Easy to use: no separation column required; isolation is achieved with a magnetic separator. High purity: isolated cell purity can reach over 95%. Excellent cell activity: negative isolation keeps magnetic beads from directly contacting target cells, so isolated cells show no abnormal activation and do not affect downstream experiments. Fast: target cells can be isolated within 15 min. |
| Intended Use | For the isolation and purification of CD8+ T cells from mouse lymph nodes, spleen, or mixed samples thereof by negative selection, for research use. |
| Principle / Technology | Negative (untouched) magnetic isolation. A biotin-labeled monoclonal antibody cocktail binds non-target cells (non-CD8+ T cells); streptavidin-labeled magnetic beads then capture and remove the labeled non-target cells on a magnetic separator, leaving bead-free mouse CD8+ T cells in the supernatant. |
| Sample Type | Mouse lymph nodes, spleen, or mixed samples thereof |
| Performance Range / Specifications | Sorting capacity: 10 T: up to 1x10^8 cells 50 T: up to 5x10^8 cells 100 T: up to 1x10^9 cells Purity: over 95% Isolation time: within 15 min Typical recovery: approximately 70-80%, depending on the starting cell number and the initial proportion of CD8+ T cells |
| Specificity | Specifically isolates mouse CD8+ T cells; the negative antibody cocktail targets non-CD8+ T cells for depletion. |
| Reaction Conditions / Protocol | Negative isolation workflow (column-free): 1. Prepare a single-cell suspension from the appropriate sample. 2. Incubate the cells with the biotin-labeled negative antibody cocktail to label non-target cells. 3. Add streptavidin magnetic beads to capture the labeled non-target cells. 4. Place the tube on a magnetic separator; non-target cells are retained by the magnet. 5. Collect the supernatant containing the untouched, bead-free target cells. The entire isolation can be completed in as little as 15 min. |
| Components / Formulation | Mouse CD8+ T Cell Negative Antibody Cocktail: 20 μL (10 T) / 100 μL (50 T) / 200 μL (100 T) Streptavidin Magnetic Beads: 0.2 mL (10 T) / 1 mL (50 T) / 2x 1 mL (100 T) |
| Storage Conditions | Store at 2-8 °C. Do not freeze. |
| Shelf Life | 1 year when stored at 2-8 °C. |
| Package Specifications | 10 T / 50 T / 100 T |
| Product Form | Liquid kit containing a biotin-labeled antibody cocktail and a streptavidin magnetic bead suspension |
| Quality Control | Purity verified by flow cytometry. In representative testing, CD3+, CD4+ and CD8+ T cells isolated from C57BL/6 mouse splenocytes with the corresponding negative isolation kits reached purities of 97.6%, 98.8% and 97.1%, respectively. |
| Purity | Isolated cell purity can reach over 95% |
| Expiration Date / Stability | Stable for 1 year when stored at 2-8 °C; do not freeze. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Designed for use with a magnetic separator; no separation column required. Best results are obtained with a 5 mL metal magnetic rack. A magnetic rack with a field strength greater than 7500 gauss is recommended. |
| Application Notes / Precautions | The number of recovered CD8+ T cells depends on the starting cell amount; the recovery rate is approximately 70-80%. Estimated yield = 10^7 cells x 15% (initial CD8+ T cell content) x 70% (recovery rate). For spleen samples from tumor-bearing or specially treated mice, the splenic cell composition may change, making the negative antibody cocktail less suitable and resulting in lower purity (about 60%); positive selection magnetic beads are recommended for such special samples. Do not freeze the kit components. |
For research use only, not for clinical use.
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