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| Product Name | Mouse CD4+ T Cell Isolation Kit |
| Catalog No. | ATR-SPS-0042 |
| Description | A magnetic negative-selection kit for enriching CD4+ T cells from mouse lymphoid tissues without directly labeling the target population. Non-target cells are tagged with biotinylated antibodies and removed using streptavidin magnetic beads. |
| Intended Use | Isolation of mouse CD4+ T cells for culture, phenotyping and functional studies. |
| Principle / Technology | Column-free negative selection using biotinylated antibodies and streptavidin-coated magnetic beads. |
| Detection Method | Magnetic cell separation followed by flow-cytometric purity assessment. |
| Sample Type | Mouse spleen, lymph node and mixed lymphoid-tissue single-cell suspensions. |
| Performance Range / Specifications | Typical separation time: approximately 16 minutes; reported purity: 96-99%; recommended processing range: 1 × 10^7 to 2 × 10^8 cells at approximately 1 × 10^8 cells/mL; package capacity: up to 1 × 10^9 cells. |
| Reaction Conditions / Protocol | Label non-target cells with the antibody cocktail, add streptavidin magnetic beads and retain the unlabeled CD4+ T-cell fraction after magnetic separation. |
| Components / Formulation | Biotinylated cell-isolation antibody cocktail and streptavidin magnetic beads. |
| Storage Conditions | Store at 2-8°C. Do not freeze. |
| Package Specifications | For up to 1 × 10^9 cells. |
| Product Form | Column-free magnetic cell-isolation kit. |
| Key Features | Approximately sixteen-minute workflow; 96-99% reported purity; bead-free target cells; no separation column; high viability and yield; no abnormal activation. |
| Shipping Conditions | Ship with ice packs. |
| Compatibility | Compatible magnetic separators, flow cytometry, cell culture and T-cell functional assays. |
| Application Notes / Precautions | Maintain the recommended cell concentration, mix beads thoroughly and keep cells cold throughout the separation procedure. |
For research use only, not for clinical use.
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