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| Product Name | Mouse CD3+ T Cell Isolation Kit |
| Catalog No. | RESK-0467 |
| Description | The Mouse CD3+ T Cell Isolation Kit (Negative Isolation) isolates and purifies CD3+ T cells from mouse lymph nodes, spleen, and mixed samples thereof by negative selection. A biotin-labeled monoclonal antibody cocktail is used to label non-target cells (non-CD3+ T cells), and streptavidin-labeled magnetic beads are then used to deplete the non-target cells, yielding mouse CD3+ T cells free of magnetic bead labeling. |
| Intended Use | For the isolation and purification of untouched mouse CD3+ T cells from mouse lymph nodes, spleen, and mixed samples thereof by negative selection. |
| Principle / Technology | Negative (column-free) magnetic selection: non-target cells are labeled with a biotin-conjugated monoclonal antibody cocktail and then depleted with streptavidin-conjugated magnetic beads using a magnetic separator, leaving the target CD3+ T cells untouched and free of bead labeling. |
| Sample Type | Mouse lymph node, spleen, and mixed samples thereof. Not recommended for PBMC separation, as the Negative Antibody Cocktail is specifically designed for mouse lymph node and spleen samples. |
| Performance Range / Specifications | Simple operation: no separation column required; target cells are isolated using a magnetic separator. High purity: sorted cell purity can reach over 95%; in flow cytometric analysis of CD3 T cells sorted from C57BL/6 mouse spleen cells, purity reached 97.6%. Good viability: with negative selection, the beads do not directly contact the target cells, so the sorted cells show no abnormal activation and downstream experiments are not affected. Fast: target cells can be sorted in 15 minutes. |
| Reaction Conditions / Protocol | Label non-target cells with the biotin-conjugated Mouse CD3+ T Cell Negative Antibody Cocktail, then add Streptavidin Magnetic Beads to deplete the labeled non-target cells using a magnetic separator. The remaining unlabeled fraction contains the purified CD3+ T cells. Target cells can be sorted within 15 minutes. |
| Components / Formulation | Mouse CD3+ T Cell Negative Antibody Cocktail: 20 uL (10 T) / 100 uL (50 T) / 200 uL (100 T) Streptavidin Magnetic Beads: 0.2 mL (10 T) / 1 mL (50 T) / 2 x 1 mL (100 T) |
| Storage Conditions | Store at 2-8 °C. Do not freeze. |
| Shelf Life | 1 year when stored at 2-8 °C. |
| Package Specifications | 10 T (up to 1×10^8 cells) / 50 T (up to 5×10^8 cells) / 100 T (up to 1×10^9 cells). |
| Product Form | Kit containing a liquid antibody cocktail and a magnetic bead suspension. |
| Purity | Sorted cell purity can reach over 95% (97.6% for CD3 T cells sorted from C57BL/6 mouse spleen cells). |
| Expiration Date / Stability | Valid for 1 year when stored at 2-8 °C; do not freeze. |
| Regulatory / Compliance | For research use only. |
| Compatibility | A magnetic separator with a magnetic field strength above 7,500 gauss is required; using a 5 mL metal magnetic stand gives better separation results. |
| Application Notes / Precautions | Do not freeze. Not recommended for PBMC separation: the Negative Antibody Cocktail is specifically designed for mouse lymph node, spleen, and mixed samples, and different sample types require different antibody cocktails. Because negative selection relies on bead-mediated depletion of non-target cells, a magnetic stand with strong magnetism (above 7,500 gauss) is recommended to ensure efficient capture of non-target cells. Best separation results are obtained when used together with a 5 mL metal magnetic stand. |
For research use only, not for clinical use.
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